BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	3,402,600	G→A	S196S (AGC→AGT)	yhdA ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	3,402,600	0	G	A	100.0%	565.2 / NA	141	S196S (AGC→AGT)	yhdA	conserved inner membrane protein
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (66/75); total (66/75)

Only 100 of 148 total aligned reads displayed.

GGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCA  >  AC_000091/3402513‑3402685

 gcggcATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCActactg                                                                                    >  1:2753915/1‑90 (MQ=255)

  cggcATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTgg                                                                                   >  2:2554237/1‑90 (MQ=255)

  cggcATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTgg                                                                                   >  1:3331282/1‑90 (MQ=255)

  cggcATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTgg                                                                                   >  2:2591593/1‑90 (MQ=255)

  cggcATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAACAGCGTATCCAGCGCACTACTgg                                                                                   >  2:229315/1‑90 (MQ=255)

   ggcATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGt                                                                                  <  2:2464067/90‑1 (MQ=255)

   ggcATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGt                                                                                  <  1:287476/90‑1 (MQ=255)

   ggcATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGt                                                                                  >  1:310083/1‑90 (MQ=255)

     cATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTc                                                                                <  1:1888671/90‑1 (MQ=255)

      aTAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCt                                                                               >  1:3063153/1‑90 (MQ=255)

       tAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTg                                                                              <  1:920325/90‑1 (MQ=255)

       tAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTg                                                                              >  2:503318/1‑90 (MQ=255)

        aaGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTgg                                                                             >  1:1705386/1‑90 (MQ=255)

        aaGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTgg                                                                             >  1:3030193/1‑90 (MQ=255)

        aaGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTgg                                                                             >  2:647892/1‑90 (MQ=255)

         agagCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTggg                                                                            >  1:466408/1‑90 (MQ=255)

         agagCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTggg                                                                            >  2:90134/1‑90 (MQ=255)

              ggATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGcc                                                                       <  1:1560580/90‑1 (MQ=255)

               gATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCa                                                                      <  2:1466649/90‑1 (MQ=255)

                aTCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCAt                                                                     >  1:241201/1‑90 (MQ=255)

                 tCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGATGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCAtt                                                                    <  1:2697197/90‑1 (MQ=255)

                 tCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCAtt                                                                    >  2:1057678/1‑90 (MQ=255)

                 tCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCAtt                                                                    <  1:2828037/90‑1 (MQ=255)

                   aGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCa                                                                  <  1:3043570/90‑1 (MQ=255)

                    gCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCAt                                                                 <  1:297842/90‑1 (MQ=255)

                    gCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCAt                                                                 <  2:1264094/90‑1 (MQ=255)

                     cGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATa                                                                <  2:330072/90‑1 (MQ=255)

                       tATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGa                                                              <  1:2115652/90‑1 (MQ=255)

                       tATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGa                                                              >  2:1221743/1‑90 (MQ=255)

                       tATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGa                                                              >  2:357833/1‑90 (MQ=255)

                        aTCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGAt                                                             <  2:235894/90‑1 (MQ=255)

                        aTCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGAt                                                             >  2:239424/1‑90 (MQ=255)

                         tCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATg                                                            <  1:2666773/90‑1 (MQ=255)

                         tCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATg                                                            <  1:3293396/90‑1 (MQ=255)

                          cAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATgg                                                           >  1:2262321/1‑90 (MQ=255)

                            aGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGtt                                                         >  1:2659323/1‑90 (MQ=255)

                             gACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTc                                                        <  2:3270632/90‑1 (MQ=255)

                                ggCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCa                                                     <  2:1663175/90‑1 (MQ=255)

                                ggCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCa                                                     >  1:2272156/1‑90 (MQ=255)

                                ggCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCa                                                     <  2:2904118/90‑1 (MQ=255)

                                ggCTGTGTTGTTCGCGTGCGATCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCa                                                     >  2:2988873/1‑90 (MQ=255)

                                 gCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCaa                                                    <  2:2567600/90‑1 (MQ=255)

                                    gtgtTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAacac                                                 >  1:595845/1‑90 (MQ=255)

                                    gtgtTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAacac                                                 >  2:156588/1‑90 (MQ=255)

                                     tgttgtTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAAcaca                                                <  1:62604/90‑1 (MQ=255)

                                       ttgttCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACAtt                                              <  1:2365966/90‑1 (MQ=255)

                                          ttCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTaga                                           <  2:1511621/90‑1 (MQ=255)

                                           tCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTagag                                          <  1:1970862/90‑1 (MQ=255)

                                            cgcgTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGc                                         >  2:2859258/1‑90 (MQ=255)

                                            cgcgTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACCGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGc                                         <  2:1195592/90‑1 (MQ=255)

                                             gcgtgcgtTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGcc                                        >  2:2695657/1‑90 (MQ=255)

                                             gcgtgcgtTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGcc                                        <  1:1098499/90‑1 (MQ=255)

                                              cgtgcgtTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCa                                       <  1:350264/90‑1 (MQ=255)

                                              cgtgcgtTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCa                                       <  2:1371056/90‑1 (MQ=255)

                                              cgtgcgtTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCa                                       <  1:1747842/90‑1 (MQ=255)

                                              cgtgcgtTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCa                                       <  2:1672871/90‑1 (MQ=255)

                                               gtgcgtTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCAc                                      <  1:1450059/90‑1 (MQ=255)

                                                tgcgtTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACg                                     >  2:3040628/1‑90 (MQ=255)

                                                tgcgtTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACg                                     <  1:2423852/90‑1 (MQ=255)

                                                tgcgtTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACg                                     >  2:2921346/1‑90 (MQ=255)

                                                  ctttCTGAATTTCACGATGCAGCGTATCCAGCGCACTACTTGTTTTGGGAGGCCATTCATAGATGGTTCCCAACTCATTAGAGCCACGCt                                   <  2:2456616/88‑1 (MQ=255)

                                                  cgtTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACCCATTAGAGCCACGCt                                   <  2:1787014/90‑1 (MQ=255)

                                                  cgtTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCt                                   <  1:855026/90‑1 (MQ=255)

                                                    ttCTGAATTTCACGAAGCAGCGTATCCTGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCa                                 <  1:3108265/90‑1 (MQ=255)

                                                    ttCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCa                                 <  2:3139943/90‑1 (MQ=255)

                                                    ttCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCa                                 >  2:3152524/1‑90 (MQ=255)

                                                     tCTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCAc                                <  2:2780563/90‑1 (MQ=255)

                                                      cTGAATTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCAcc                               <  2:1440291/90‑1 (MQ=255)

                                                         aaTTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGtt                            <  1:3221262/90‑1 (MQ=255)

                                                         aaTTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGtt                            <  2:2693869/90‑1 (MQ=255)

                                                         aaTTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGtt                            <  2:638722/90‑1 (MQ=255)

                                                         aaTTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGtt                            <  1:1455823/90‑1 (MQ=255)

                                                          aTTTCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGttt                           <  2:1330894/90‑1 (MQ=255)

                                                             tCACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAg                        >  1:1918846/1‑90 (MQ=255)

                                                              cACGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGa                       <  1:2646163/90‑1 (MQ=255)

                                                               aCGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGAt                      <  1:703372/90‑1 (MQ=255)

                                                               aCGAAGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTGCCCAACAGATTAGAGCCACGCTCACCGTTTAAGAt                      >  1:2981781/1‑90 (MQ=255)

                                                                  aaGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACg                   <  1:1024488/90‑1 (MQ=255)

                                                                  aaGCAGCGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACg                   <  1:2264464/90‑1 (MQ=255)

                                                                   agcagcGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGa                  >  1:1705877/1‑90 (MQ=255)

                                                                     cagcGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCGACGCTCACCGTTTAAGATACGAGt                >  1:3400103/1‑90 (MQ=255)

                                                                     cagcGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGt                <  2:1888745/90‑1 (MQ=255)

                                                                      agcGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTa               >  2:2127520/1‑90 (MQ=255)

                                                                      agcGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTa               <  1:3481362/90‑1 (MQ=255)

                                                                      agcGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTa               <  2:3029483/90‑1 (MQ=255)

                                                                       gcGTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAg              <  1:876273/90‑1 (MQ=255)

                                                                         gTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGcc            >  1:2569504/1‑90 (MQ=255)

                                                                         gTATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGcc            >  1:60551/1‑90 (MQ=255)

                                                                          tATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCCCCGTTTAAGATACGAGTAGccc           <  1:1061116/90‑1 (MQ=255)

                                                                          tATCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGccc           <  1:2779731/90‑1 (MQ=255)

                                                                           aTCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCg          >  1:30065/1‑90 (MQ=255)

                                                                           aTCCAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCg          >  2:360874/1‑90 (MQ=255)

                                                                              cAGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGt       >  1:2323270/1‑90 (MQ=255)

                                                                               aGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGtt      >  1:403061/1‑90 (MQ=255)

                                                                               aGCGCACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGtt      <  2:1944518/90‑1 (MQ=255)

                                                                                gcgcACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGttt     >  2:2030524/1‑90 (MQ=255)

                                                                                 cgcACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTc    >  2:590607/1‑90 (MQ=255)

                                                                                  gcACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTcc   >  2:2782838/1‑90 (MQ=255)

                                                                                  gcACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTcc   <  2:707993/90‑1 (MQ=255)

                                                                                   cACTACTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCa  >  1:1566134/1‑90 (MQ=255)

GGCGGCATAAGAGCGGATCAGCGTATCAAGACGGCTGTGTTGTTCGCGTGCGTTCTGAATTTCACGAAGCAGCGTATCCAGCGCACTGCTGGTTCTGGGCGGCCATTCATAGATGGTTCCCAACACATTAGAGCCACGCTCACCGTTTAAGATACGAGTAGCCCGGGTTTCCA  >  AC_000091/3402513‑3402685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`