BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AC_000091	3,557,661	A→G	intergenic (‑271/+582)	yiiE ← / ← yiiD	predicted transcriptional regulator/predicted acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AC_000091	3,557,661	0	A	G	100.0%	492.6 / NA	122	intergenic (‑271/+582)	yiiE/yiiD	predicted transcriptional regulator/predicted acetyltransferase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (67/55); total (67/55)

Only 100 of 124 total aligned reads displayed.

TATGGGTGCTATGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCACCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCTTTACTTGCTCATCGTTATAATTT  >  AC_000091/3557574‑3557747

tATGGGTGCTATGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGcc                                                                                      <  2:1608435/90‑1 (MQ=255)

     gTGCTATGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGAt                                                                                 >  2:3392799/1‑90 (MQ=255)

     gTGCTATGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGAt                                                                                 <  2:2871058/90‑1 (MQ=255)

      tGCTATGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGAtt                                                                                >  2:1517118/1‑90 (MQ=255)

      tGCTATGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGAtt                                                                                <  2:1083370/90‑1 (MQ=255)

      tGCTATGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGCAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGAtt                                                                                >  1:2807230/1‑90 (MQ=255)

       gCTATGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGAttt                                                                               <  1:771179/90‑1 (MQ=255)

       gCTATGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGAttt                                                                               >  1:1229756/1‑90 (MQ=255)

         tATGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTaa                                                                             <  1:810933/90‑1 (MQ=255)

         tATGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTaa                                                                             <  1:1378278/90‑1 (MQ=255)

          aTGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAg                                                                            <  1:975586/90‑1 (MQ=255)

          aTGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAg                                                                            <  1:389755/90‑1 (MQ=255)

           tGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCGTCCTAACAGCGCCAGGATTTAAgg                                                                           >  1:524773/1‑90 (MQ=255)

           tGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAgg                                                                           <  1:1910784/90‑1 (MQ=255)

           tGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAgg                                                                           >  2:2101923/1‑90 (MQ=255)

           tGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGCGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAgg                                                                           >  2:2432731/1‑90 (MQ=255)

            gAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGt                                                                          <  1:1958935/90‑1 (MQ=255)

            gAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGt                                                                          <  1:1926372/90‑1 (MQ=255)

                atattatattCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGaaa                                                                      >  2:117695/1‑90 (MQ=255)

                atattatattCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGaaa                                                                      >  2:3004416/1‑90 (MQ=255)

                 tattatattCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAAt                                                                     >  2:1621045/1‑90 (MQ=255)

                 tattatattCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAAt                                                                     <  1:915258/90‑1 (MQ=255)

                 tattatattCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAAt                                                                     >  1:915198/1‑90 (MQ=255)

                 tattatattCAGAGATATTTATTTGCAAAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAAt                                                                     >  2:2779464/1‑90 (MQ=255)

                  attatattCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAAtt                                                                    >  1:2598390/1‑90 (MQ=255)

                  attatattCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAAtt                                                                    <  1:1662003/90‑1 (MQ=255)

                  attatattCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAAtt                                                                    >  2:65013/1‑90 (MQ=255)

                   ttatattCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTa                                                                   <  2:1795513/90‑1 (MQ=255)

                    tatattCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTaa                                                                  >  2:1205429/1‑90 (MQ=255)

                    tatattCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTaa                                                                  <  2:3083058/90‑1 (MQ=255)

                    tatattCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTaa                                                                  >  1:1509118/1‑90 (MQ=255)

                     atattCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAAt                                                                 >  2:53229/1‑90 (MQ=255)

                      tattCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATc                                                                <  2:860090/90‑1 (MQ=255)

                      tattCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATc                                                                <  1:1644256/90‑1 (MQ=255)

                        ttCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCTTAACAGCGCCAGGATTTAAGGTGAAATTAATCtt                                                              >  2:2740793/1‑90 (MQ=255)

                        ttCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCtt                                                              >  1:1948441/1‑90 (MQ=255)

                        ttCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCtt                                                              >  1:1939589/1‑90 (MQ=255)

                         tCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCttt                                                             >  1:2029965/1‑90 (MQ=255)

                          cAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTtc                                                            <  1:3016263/90‑1 (MQ=255)

                          cAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTtc                                                            <  2:151016/90‑1 (MQ=255)

                          cAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTtc                                                            >  2:2426997/1‑90 (MQ=255)

                          cAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTtc                                                            >  2:2867062/1‑90 (MQ=255)

                           agagATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTtca                                                           <  2:1779064/90‑1 (MQ=255)

                            gagaTATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTtcat                                                          <  2:534733/90‑1 (MQ=255)

                             agaTATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTtcatc                                                         >  2:2068883/1‑90 (MQ=255)

                              gaTATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTtcatca                                                        <  1:1021643/90‑1 (MQ=255)

                               atatTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATcac                                                       >  1:1295161/1‑90 (MQ=255)

                                tatttattTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATcaca                                                      <  1:3034022/90‑1 (MQ=255)

                                tatttattTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATcaca                                                      >  1:2989768/1‑90 (MQ=255)

                                    tatttGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGAcc                                                  <  1:997250/90‑1 (MQ=255)

                                     atttGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCg                                                 <  1:2801313/90‑1 (MQ=255)

                                       ttGCATAATCTGAATGGTGTAGCCGGAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGt                                               >  2:278736/1‑90 (MQ=255)

                                       ttGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGt                                               <  1:2716971/90‑1 (MQ=255)

                                       ttGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGt                                               <  1:1539393/90‑1 (MQ=255)

                                        tGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTc                                              <  1:2846061/90‑1 (MQ=255)

                                        tGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTc                                              <  2:316962/90‑1 (MQ=255)

                                         gCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATGTTTCATCACAGACCGGTCa                                             >  1:3413363/1‑90 (MQ=255)

                                         gCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCa                                             <  1:2466056/90‑1 (MQ=255)

                                         gCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCa                                             <  2:1938330/90‑1 (MQ=255)

                                           aTAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGt                                           >  1:2344935/1‑90 (MQ=255)

                                              aTCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACt                                        <  2:3216676/90‑1 (MQ=255)

                                              aTCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACt                                        >  1:3227233/1‑90 (MQ=255)

                                               tCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTg                                       >  2:466329/1‑90 (MQ=255)

                                               tCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTg                                       <  2:761193/90‑1 (MQ=255)

                                               tCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTg                                       <  2:1463958/90‑1 (MQ=255)

                                                cTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGc                                      >  1:1267008/1‑90 (MQ=255)

                                                 tGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGcc                                     >  1:706987/1‑90 (MQ=255)

                                                  gAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGcca                                    <  1:3243271/90‑1 (MQ=255)

                                                   aaTGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGccac                                   >  1:2535515/1‑90 (MQ=255)

                                                     tGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGccacca                                 <  2:827153/90‑1 (MQ=255)

                                                     tGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGccacca                                 >  1:1540326/1‑90 (MQ=255)

                                                      ggTGTAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCaa                                >  1:1066712/1‑90 (MQ=255)

                                                       gtgtAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAAc                               >  2:1156179/1‑90 (MQ=255)

                                                         gtAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCg                             >  1:3426819/1‑90 (MQ=255)

                                                         gtAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCg                             >  2:1971006/1‑90 (MQ=255)

                                                         gtAGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGGGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCg                             >  1:2519165/1‑90 (MQ=255)

                                                           aGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGt                           >  2:1901708/1‑90 (MQ=255)

                                                           aGCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGt                           <  2:2607294/90‑1 (MQ=255)

                                                            gCCGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTc                          <  2:2014063/90‑1 (MQ=255)

                                                              cGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCtt                        <  2:3401589/90‑1 (MQ=255)

                                                              cGAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCtt                        >  2:2841217/1‑90 (MQ=255)

                                                               gAAAAACGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCttt                       >  2:1775293/1‑90 (MQ=255)

                                                                 aaaaCGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCTTTAc                     >  1:2014300/1‑90 (MQ=255)

                                                                 aaaaCGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCTTTAc                     <  2:2427723/90‑1 (MQ=255)

                                                                  aaaCGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCCCAGACCGGTCAGTACTGCCACCAACCGGTCTTTACt                    <  2:1486484/90‑1 (MQ=255)

                                                                   aaCGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCTTTACtt                   <  1:831286/90‑1 (MQ=255)

                                                                   aaCGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCTTTACtt                   <  1:676364/90‑1 (MQ=255)

                                                                    aCGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCTTTACTTg                  <  2:835526/90‑1 (MQ=255)

                                                                     cGTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCTTTACTTGc                 >  2:164812/1‑90 (MQ=255)

                                                                      gTCAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACGGGTCTTTACTTGCt                >  2:2599871/1‑90 (MQ=255)

                                                                        cAGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCTTTACTTGCTCa              >  2:194064/1‑90 (MQ=255)

                                                                         aGCATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCTTTACTTGCTCAt             >  2:1629479/1‑90 (MQ=255)

                                                                           cATCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCTTTACTTGCTCATCg           >  1:1675600/1‑90 (MQ=255)

                                                                           cATCCTAACAGCGCCAGGACTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCTTTACTTGCTCATCg           <  1:3021444/90‑1 (MQ=255)

                                                                            aTCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCCGTAATGCCACCCACCGGTCTCTACTTGCTCATCGt          >  1:675434/1‑90 (MQ=255)

                                                                             tCCTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCTTTACTTGCTCATCGtt         <  1:2903042/90‑1 (MQ=255)

                                                                               cTAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTTATCACAGACCGGTCAGTTCTGCCACCAACCGGTCTTTACATGCTCATCGTtat       <  2:2198678/90‑1 (MQ=255)

                                                                                tAACAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCTTTACTTGCTCATCGTtata      >  1:2139296/1‑90 (MQ=255)

                                                                                  aCAGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGGACTGCCACCAACCGGTCTTTACTTGCTCATCGTTATAAt    >  2:176930/1‑90 (MQ=255)

                                                                                    aGCGCCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCTTTACTTGCTCATCGTTATAAttt  <  2:1144679/90‑1 (MQ=255)

TATGGGTGCTATGAATATATTATATTCAGAGATATTTATTTGCATAATCTGAATGGTGTAGCCGAAAAACGTCAGCATCCTAACAGCACCAGGATTTAAGGTGAAATTAATCTTTCATCACAGACCGGTCAGTACTGCCACCAACCGGTCTTTACTTGCTCATCGTTATAATTT  >  AC_000091/3557574‑3557747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`