Predicted mutation
evidence seq id position mutation annotation gene description
RA AC_000091 3,624,613 T→C L250P (CTG→CCG)  metR → DNA‑binding transcriptional activator, homocysteine‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AC_0000913,624,6130TC100.0% 441.1 / NA 115L250P (CTG→CCG) metRDNA‑binding transcriptional activator, homocysteine‑binding
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (56/59);  total (56/59)

Only 100 of 123 total aligned reads displayed.

CCTTATTGTTGATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCTGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCAGCCAGTTACG  >  AC_000091/3624526‑3624698
                                                                                       |                                                                                     
ccTTATTGTTGATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCcgg                                                                                     >  1:3142948/1‑90 (MQ=255)
 cTTATTGTTGATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCcggt                                                                                    >  2:1227105/1‑90 (MQ=255)
 cTTATTGTTGATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCcggt                                                                                    <  1:1882750/90‑1 (MQ=255)
  ttATTGTTGATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCcggtg                                                                                   >  2:1758613/1‑90 (MQ=255)
  ttATTGTTGATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCcggtg                                                                                   <  2:1533607/90‑1 (MQ=255)
   tATTGTTGATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCcggtgg                                                                                  >  1:2586351/1‑90 (MQ=255)
    aTTGTTGATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGGGAGTTTTGAGCGCCAGGGTCCggtggt                                                                                 >  2:361856/1‑90 (MQ=255)
     ttgttgATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGgtggtg                                                                                >  1:1639455/1‑90 (MQ=255)
      tgttgATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGa                                                                               >  2:1429998/1‑90 (MQ=255)
       gttgATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGAc                                                                              <  2:2924923/90‑1 (MQ=255)
       gttgATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGAc                                                                              >  1:2471400/1‑90 (MQ=255)
       gttgATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGAc                                                                              <  1:2421632/90‑1 (MQ=255)
        ttgATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGAcc                                                                             >  1:3165156/1‑90 (MQ=255)
        ttgATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGAcc                                                                             >  2:1442047/1‑90 (MQ=255)
         tgATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCa                                                                            >  2:1774432/1‑90 (MQ=255)
         tgATTCAGATGGTTGCCGCGCGGATAGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCa                                                                            <  1:359002/90‑1 (MQ=255)
          gATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCaa                                                                           <  2:3363491/90‑1 (MQ=255)
          gATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCaa                                                                           >  1:793777/1‑90 (MQ=255)
           aTTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCaaa                                                                          <  1:643070/90‑1 (MQ=255)
           aTTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCaaa                                                                          <  2:1422493/90‑1 (MQ=255)
             tCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAAc                                                                        <  1:2049660/90‑1 (MQ=255)
              cAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAAcc                                                                       >  1:3235590/1‑90 (MQ=255)
              cAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAAcc                                                                       <  2:2652714/90‑1 (MQ=255)
               aGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAAccc                                                                      >  2:1049072/1‑90 (MQ=255)
               aGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAAccc                                                                      >  2:2413940/1‑90 (MQ=255)
                gATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCt                                                                     >  2:2464197/1‑90 (MQ=255)
                gATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCt                                                                     <  2:2961026/90‑1 (MQ=255)
                gATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCt                                                                     >  2:715777/1‑90 (MQ=255)
                 aTGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTg                                                                    <  1:1133316/90‑1 (MQ=255)
                 aTGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTg                                                                    <  2:2421350/90‑1 (MQ=255)
                  tGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTgg                                                                   >  1:2905571/1‑90 (MQ=255)
                  tGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTgg                                                                   <  2:3288261/90‑1 (MQ=255)
                    gTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGc                                                                 >  1:3443231/1‑90 (MQ=255)
                    gTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGc                                                                 >  2:347209/1‑90 (MQ=255)
                     ttGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCg                                                                <  1:2159354/90‑1 (MQ=255)
                     ttGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCg                                                                <  2:1156025/90‑1 (MQ=255)
                      tGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGa                                                               >  2:2223/1‑90 (MQ=255)
                      tGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGa                                                               >  2:2984820/1‑90 (MQ=255)
                       gCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGaa                                                              >  1:596224/1‑90 (MQ=255)
                        ccGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAGAACCCTGGGCGAAg                                                             <  1:985348/90‑1 (MQ=255)
                           cgcgGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCt                                                          >  1:3261463/1‑90 (MQ=255)
                           cgcgGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCt                                                          <  2:2587445/90‑1 (MQ=255)
                              ggATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTtgt                                                       >  1:1088088/1‑90 (MQ=255)
                              ggATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTtgt                                                       <  1:2149996/90‑1 (MQ=255)
                                  gggTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAg                                                   <  1:2843236/90‑1 (MQ=255)
                                   ggTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGc                                                  <  2:2549942/90‑1 (MQ=255)
                                    gTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGcc                                                 >  2:657353/1‑90 (MQ=255)
                                    gTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGcc                                                 >  1:1731673/1‑90 (MQ=255)
                                     tATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCg                                                >  2:1481626/1‑90 (MQ=255)
                                      aTTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGa                                               >  2:800101/1‑90 (MQ=255)
                                      aTTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGa                                               <  2:466577/90‑1 (MQ=255)
                                        tGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACt                                             <  2:3200285/90‑1 (MQ=255)
                                        tGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACt                                             >  2:2137016/1‑90 (MQ=255)
                                             cgcTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTAcg                                        >  1:747628/1‑90 (MQ=255)
                                              gcTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTAcgc                                       >  1:1831097/1‑90 (MQ=255)
                                                tACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTAcgccg                                     >  1:10216/1‑90 (MQ=255)
                                                tACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTAcgccg                                     >  1:1921668/1‑90 (MQ=255)
                                                tACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTAcgccg                                     <  1:3009237/90‑1 (MQ=255)
                                                tACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGGCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTAcgccg                                     >  2:486043/1‑90 (MQ=255)
                                                  ccGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACgccgcc                                   <  2:150920/90‑1 (MQ=255)
                                                  ccGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACgccgcc                                   >  1:2484799/1‑90 (MQ=255)
                                                   cGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGccgccg                                  >  1:1754361/1‑90 (MQ=255)
                                                   cGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGccgccg                                  >  1:762908/1‑90 (MQ=255)
                                                    gCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGt                                 <  1:1100588/90‑1 (MQ=255)
                                                      aTTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTgc                               >  2:276917/1‑90 (MQ=255)
                                                       ttGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTgcg                              <  1:541439/90‑1 (MQ=255)
                                                        tGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTgcgc                             >  2:2670095/1‑90 (MQ=255)
                                                          ggTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGa                           <  2:1974138/90‑1 (MQ=255)
                                                          ggTAGTAGAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGa                           <  1:2459760/90‑1 (MQ=255)
                                                             agtagAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATgg                        <  2:1107100/90‑1 (MQ=255)
                                                              gtagAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGc                       <  2:3334358/90‑1 (MQ=255)
                                                               tagAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGATGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCg                      <  1:3376393/90‑1 (MQ=255)
                                                               tagAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCg                      >  1:2932519/1‑90 (MQ=255)
                                                               tagAGAGTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCg                      <  2:2188466/90‑1 (MQ=255)
                                                                agagagTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGa                     <  1:2307284/90‑1 (MQ=255)
                                                                 gagagTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGag                    >  1:1534862/1‑90 (MQ=255)
                                                                  agagTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGagc                   >  1:2862376/1‑90 (MQ=255)
                                                                    agTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATTGCGagcag                 >  1:580472/1‑90 (MQ=255)
                                                                    agTTTTGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGagcag                 <  1:991749/90‑1 (MQ=255)
                                                                      ttttGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCg               >  2:2348861/1‑90 (MQ=255)
                                                                      ttttGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCg               <  1:1830445/90‑1 (MQ=255)
                                                                       tttGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGt              <  2:701478/90‑1 (MQ=255)
                                                                        ttGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTc             <  1:1022488/90‑1 (MQ=255)
                                                                        ttGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTc             <  1:2422003/90‑1 (MQ=255)
                                                                         tGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCa            <  2:1175982/90‑1 (MQ=255)
                                                                         tGAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCa            <  2:747628/90‑1 (MQ=255)
                                                                          gAGCGCCAGGGTCCGGTGGTGACGAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCAg           <  2:3006945/90‑1 (MQ=255)
                                                                          gAGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCAg           <  1:3190038/90‑1 (MQ=255)
                                                                           aGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCAGc          >  1:1847814/1‑90 (MQ=255)
                                                                           aGCGCCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCAGc          >  1:271523/1‑90 (MQ=255)
                                                                             cgcCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCAGCCa        <  2:1724935/90‑1 (MQ=255)
                                                                              gcCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCAGCCAg       >  2:194540/1‑90 (MQ=255)
                                                                              gcCAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCAGCCAg       <  2:1943272/90‑1 (MQ=255)
                                                                               ccAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCAGCCAGt      <  2:2246001/90‑1 (MQ=255)
                                                                               ccAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCAGCCAGt      >  2:2672692/1‑90 (MQ=255)
                                                                                cAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCAGCCAGtt     >  1:1526372/1‑90 (MQ=255)
                                                                                cAGGGTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCAGCCAGtt     >  2:1033011/1‑90 (MQ=255)
                                                                                  gggTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCAGCCAGTTAc   <  1:623680/90‑1 (MQ=255)
                                                                                  gggTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCAGCCAGTTAc   <  2:117050/90‑1 (MQ=255)
                                                                                   ggTCCGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCAGCCAGTTACg  <  1:2493649/90‑1 (MQ=255)
                                                                                       |                                                                                     
CCTTATTGTTGATTCAGATGGTTGCCGCGCGGATGGGTATTGCCGCGCTACCGCATTGGGTAGTAGAGAGTTTTGAGCGCCAGGGTCTGGTGGTGACCAAAACCCTGGGCGAAGGCTTGTGGAGCCGACTGTACGCCGCCGTGCGCGATGGCGAGCAGCGTCAGCCAGTTACG  >  AC_000091/3624526‑3624698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑