New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NZ_CP009273	281585 =	59 (2.770)	5 (0.260)	4/152	1.2	9.3%	coding (480/1383 nt)	yagG	MFS transporter
	?	NZ_CP009273	281607 =	44 (2.280)					coding (502/1383 nt)	yagG	MFS transporter
Rejected: Frequency below/above cutoff threshold.

CCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP009273/281682‑281585 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgccgctgGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCT  >  NZ_CP009273/281607‑281689                                                                                                                                                                                       CCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCCCGAT                                                                                            <  2:581505/91‑1  CACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATG                                                                                           <  1:450247/91‑1  CACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATG                                                                                           <  1:82520/91‑1   ACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGC                                                                                          >  1:337788/1‑91   ACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGC                                                                                          >  2:538352/1‑91   ACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGC                                                                                          >  2:413636/1‑91   ACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGC                                                                                          >  2:181363/1‑91   ACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGC                                                                                          >  2:244960/1‑91        GCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTG                                                                                     >  2:507780/1‑91        GCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTG                                                                                     >  2:299001/1‑91                       GCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGC                                                                                       <  2:561807/74‑1                       GCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGC                                                                                       >  1:485065/1‑74                       GCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGC                                                                                       >  1:561807/1‑74                       GCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGC                                                                                       <  2:485065/74‑1                            TGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATG                                                                                           <  1:252028/65‑1                            TGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATG                                                                                           >  2:252028/1‑65                                       TAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGG                                                      <  1:93348/91‑1                                        AGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGT                                                     <  1:529095/91‑1                                        AGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGT                                                     <  1:191907/91‑1                                             ACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGC                                                                                       >  1:544635/1‑52                                             ACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGC                                                                                       <  2:544635/52‑1                                                         CCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCA                                    <  1:404159/91‑1                                                              GCCGATGATCCTCACCAGCGGCAGCGCGATGCCGCGGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGC                               <  2:69371/91‑1                                                                                           TGCCGCTGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCT  <  2:615719/91‑1                                                                                                 TGGTGAGCATCATCGGCAAAGGGGATGAGCAGGTGGGCTACTTCGGCGCCATGTGC                               >  2:596390/1‑56                                                                                                 TGGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGC                               <  1:596390/56‑1                                                                                                                                                                                       CCACGCCGCTCAGCCCCAGCACGCACATGGCGCCGAAGTAGCCCACCTGCTCGTCCCCTTTGCCGATGATGCTCACCAGCGGCAGCGCGATGCCGCTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NZ_CP009273/281682‑281585 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tgccgctgGTGAGCATCATCGGCAAAGGGGACGAGCAGGTGGGCTACTTCGGCGCCATGTGCGTGCTGGGGCTGAGCGGCGTGGTGCTGCT  >  NZ_CP009273/281607‑281689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.