Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	310	(T)9→8	intergenic (+55/‑27)	thrL → / → thrA	thr operon leader peptide/bifunctional aspartate kinase/homoserine dehydrogenase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	302	0	T	.	100.0%	91.4 / NA	21	intergenic (+47/‑35)	thrL/thrA	thr operon leader peptide/bifunctional aspartate kinase/homoserine dehydrogenase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (10/11);  total (10/11)

CCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTC  >  NZ_CP009273/218‑388                                                                                     |                                                                                       ccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCttttttt                                                                                  >  1:38509/1‑91 (MQ=255) ccaccaTCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTCCGGGCttgtttt                                                                                  >  2:680082/1‑91 (MQ=255)  caccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCtttttttt                                                                                 >  2:641612/1‑91 (MQ=255)  caccatcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCtttttttt                                                                                 >  1:76165/1‑91 (MQ=255)      atcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCttttttttcgac                                                                             >  1:98468/1‑87 (MQ=255)       tcaccatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGAcc                                                                           <  1:478390/91‑1 (MQ=255)         accatTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCaa                                                                         <  1:258608/91‑1 (MQ=255)            atTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAgg                                                                      >  1:131510/1‑91 (MQ=255)                 cacaGGTAACGGTGCGGGCTGACGCGTACAGGACACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACg                                                                 <  1:255353/91‑1 (MQ=255)                 cacaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACg                                                                 <  2:25514/91‑1 (MQ=255)                  acaGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGa                                                                <  2:211113/91‑1 (MQ=255)                    aGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAgg                                                              >  1:683472/1‑91 (MQ=255)                             tGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCa                                                     >  2:815842/1‑91 (MQ=255)                             tGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCa                                                     >  1:159403/1‑91 (MQ=255)                               cGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATg                                                   >  1:385386/1‑91 (MQ=255)                                      aCGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAgtgt                                            >  2:575756/1‑91 (MQ=255)                                              aGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTc                                    <  2:756841/91‑1 (MQ=255)                                              aGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTc                                    <  1:587138/91‑1 (MQ=255)                                                 aaaCAAAGAAAAACTGCCGCTCCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTcggc                                 <  1:1317/91‑1 (MQ=255)                                                    cacaGAAAAAAGCCCGCACCTGACCGGGCGGGCTTTTTTTTTGGCCCAAGGGAAAC‑AGGGAACAAACCAGGGAGGGTTGAAGTTCGGGGGt                              >  2:7256/1‑91 (MQ=255)                                                           aaaaGCCCGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAg                       <  1:815842/91‑1 (MQ=255)                                                                  cGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATg                                                   >  2:38508/1‑56 (MQ=255)                                                                  cGCACCTGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATg                                                   <  1:38508/56‑1 (MQ=255)                                                                        tGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGa          <  2:94156/91‑1 (MQ=255)                                                                        tGACAGTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGGGGCAAAAGCAGa          >  2:431986/1‑91 (MQ=255)                                                                             gTGCGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGtt     >  2:336848/1‑91 (MQ=255)                                                                                cGGGC‑TTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTc  >  1:847519/1‑91 (MQ=255)                                                                                     |                                                                                       CCACCATCACCATTACCACAGGTAACGGTGCGGGCTGACGCGTACAGGAAACACAGAAAAAAGCCCGCACCTGACAGTGCGGGCTTTTTTTTTCGACCAAAGGTAACGAGGTAACAACCATGCGAGTGTTGAAGTTCGGCGGTACATCAGTGGCAAATGCAGAACGTTTTC  >  NZ_CP009273/218‑388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑