Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,718,241	Δ1 bp	coding (1864/2013 nt)	ydhK →	FUSC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,718,240	0	G	.	100.0%	122.0 / NA	28	coding (1863/2013 nt)	ydhK	FUSC family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (18/10);  total (18/10)

TTATCGCGAGTACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGCGGCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTG  >  NZ_CP009273/1718160‑1718326                                                                                 |                                                                                       ttATCGCGAGTACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTga                                                                             <  2:431835/91‑1 (MQ=255)    tCGCGAGTACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTgaaga                                                                          <  2:531434/91‑1 (MQ=255)          gTACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACa                                                                    >  1:410606/1‑91 (MQ=255)          gTACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACa                                                                    >  1:689174/1‑91 (MQ=255)           tACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAg                                                                   <  2:631430/91‑1 (MQ=255)                        tATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTggcgcc                                                                                      <  1:261445/60‑3 (MQ=255)                        tATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTggcgcc                                                                                      >  2:261445/1‑58 (MQ=255)                         aTTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAg                                                     >  2:700808/1‑91 (MQ=255)                               ctgctgCGGGGAGCGAGGAGTGAGCGTGGCGTTCAGCAACAATCACTGGC‑GCAACACTTGAAGAATTACAGCGGATTTGCGAGAGCCTTGc                                               >  2:224056/1‑91 (MQ=255)                                tGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGcc                                              <  1:761623/91‑1 (MQ=255)                                     cGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGtca                                         <  1:23137/91‑1 (MQ=255)                                      ggggAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGtcat                                        >  2:794367/1‑91 (MQ=255)                                         gAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGtcatcat                                     >  1:677940/1‑91 (MQ=255)                                         gAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGtcatcat                                     >  1:297964/1‑91 (MQ=255)                                         gAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGtcatcat                                     >  2:438867/1‑91 (MQ=255)                                           gTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGtcatcatca                                   >  2:163203/1‑91 (MQ=255)                                           gTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGtcatcatca                                   >  2:639319/1‑91 (MQ=255)                                           gTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGtcatcatca                                   >  1:667354/1‑91 (MQ=255)                                             gatgaGTGAGCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAAc                                 >  2:347548/1‑91 (MQ=255)                                                  gtgagCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTgcc                            <  1:438867/91‑1 (MQ=255)                                                   tgagCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTgccg                           >  1:584971/1‑91 (MQ=255)                                                   tgagCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTgccg                           >  1:334935/1‑91 (MQ=255)                                                   tgagCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTgccg                           >  1:326372/1‑91 (MQ=255)                                                    gagCGTGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTgccgc                          >  2:705875/1‑91 (MQ=255)                                                         tGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTg                     <  1:794367/91‑1 (MQ=255)                                                         tGGCGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTg                     >  1:518708/1‑91 (MQ=255)                                                            cGTTCAGCAAAAATCACTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGc                  >  2:781060/1‑91 (MQ=255)                                                                       aaTCACTGGC‑GCCACACTTGAAGAATTACTGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAAtt       >  2:530449/1‑91 (MQ=255)                                                                            cTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTg  <  1:742050/91‑1 (MQ=255)                                                                            cTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTg  <  1:72546/91‑1 (MQ=255)                                                                            cTGGC‑GCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTg  <  1:60140/91‑1 (MQ=255)                                                                                 |                                                                                       TTATCGCGAGTACGGGATAACTGTATTTCACTGTTGCGGGGAGTGATGAGTGAGCGTGGCGTTCAGCAAAAATCACTGGCGGCCACACTTGAAGAATTACAGCGGATTTGCGACAGCCTTGCCCGTCATCATCAACCTGCCGCCCGTGAGCTGGCGGCAATTGTCTG  >  NZ_CP009273/1718160‑1718326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑