Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,732,351	(A)7→6	coding (251/1026 nt)	purR →	HTH‑type transcriptional repressor PurR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,732,345	0	A	.	100.0%	100.3 / NA	23	coding (245/1026 nt)	purR	HTH‑type transcriptional repressor PurR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (5/18);  total (5/18)

GCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATC  >  NZ_CP009273/1732258‑1732422                                                                                        |                                                                              gCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGaaaa                                                                            >  2:584225/1‑91 (MQ=255)              ccACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGa                                                              <  2:37018/91‑1 (MQ=255)              ccACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGa                                                              <  1:83889/91‑1 (MQ=255)               cacaCCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGaa                                                             <  2:490316/91‑1 (MQ=255)                acacCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGaaa                                                            <  1:231574/91‑1 (MQ=255)                    cAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGtt                                                        <  1:386960/91‑1 (MQ=255)                      aGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTac                                                      >  2:499228/1‑91 (MQ=255)                           aTCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCt                                                 <  1:391503/91‑1 (MQ=255)                           aTCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCt                                                 <  1:39169/91‑1 (MQ=255)                           aTCGGTCTGCGGGCGACCAGCAGCGAAGCGCCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACCCCCt                                                 <  2:555590/91‑1 (MQ=255)                             cGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAACCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGa                                               <  1:204569/91‑1 (MQ=255)                                tttGCTTGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTc                                            <  2:535291/91‑1 (MQ=255)                                   gCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTgg                                         >  1:570697/1‑91 (MQ=255)                                       gCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCaa                                     <  1:611614/91‑1 (MQ=255)                                       gCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCaa                                     <  2:72489/91‑1 (MQ=255)                                         gACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATg                                   >  1:252964/1‑91 (MQ=255)                                            cagcagCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGt                                <  1:602290/91‑1 (MQ=255)                                            cagcagCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGt                                <  1:69209/91‑1 (MQ=255)                                             agcagcGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTg                               >  1:582831/1‑91 (MQ=255)                                                 gcGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAAc                           <  1:627689/91‑1 (MQ=255)                                                                        aTCATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTa    <  2:818837/91‑1 (MQ=255)                                                                          cATTGAAGCAGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATc  <  1:499228/91‑1 (MQ=255)                                                                                   aGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCt                                                 <  2:539436/35‑1 (MQ=38)                                                                                   aGTTG‑AAAAAATTGCTTCCAGAAAGGTTACACCCt                                                 >  1:539436/1‑35 (MQ=38)                                                                                        |                                                                              GCCTGAAGGTTAACCACACCAAGTCTATCGGTTTGCTGGCGACCAGCAGCGAAGCGGCCTATTTTGCCGAGATCATTGAAGCAGTTGAAAAAAATTGCTTCCAGAAAGGTTACACCCTGATTCTGGGCAATGCGTGGAACAATCTTGAGAAACAGCGGGCTTATC  >  NZ_CP009273/1732258‑1732422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑