Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,262,978	(C)5→4	coding (822/2823 nt)	uvrA ←	excinuclease ABC subunit UvrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,262,974	0	C	.	100.0%	99.0 / NA	23	coding (826/2823 nt)	uvrA	excinuclease ABC subunit UvrA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (14/9);  total (14/9)

CCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGG  >  NZ_CP009273/4262889‑4263057                                                                                      |                                                                                    ccagccagCGACAGTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGccccgc                                                                                >  2:271182/1‑89 (MQ=255)           tcAGTTCCGGATTCTGGATCACTCGATCAGGTTCGGAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCGGCCGGGTTGTTa                                                                     >  2:849975/2‑91 (MQ=255)              gTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAAc                                                                  >  2:710565/1‑91 (MQ=255)              gTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAAc                                                                  >  2:353927/1‑91 (MQ=255)              gTTCCGGATTCTGGATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAAc                                                                  >  2:240650/1‑91 (MQ=255)               ttCCGGATTCTGGATCACTCGAGCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACg                                                                 <  1:137812/91‑1 (MQ=255)                           gATCACTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTcgcg                                                     <  1:831081/91‑1 (MQ=255)                               aCTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTc                                                 >  2:272320/1‑91 (MQ=255)                               aCTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTc                                                 >  2:830110/1‑91 (MQ=255)                               aCTCGATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTc                                                 >  2:667889/1‑91 (MQ=255)                                   gATCAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGt                                             <  1:461022/91‑1 (MQ=255)                                      cAGGTTCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCa                                          <  1:176305/91‑1 (MQ=255)                                        ggttCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACg                                        <  1:526999/88‑1 (MQ=255)                                          ttCGAAATATTGCTGTACGCCAAGGCCGTCGCCGGTCGGGCACG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCCGCTCCAGTTCACGct                                      >  2:679733/2‑90 (MQ=255)                                                        gTACGCCAAGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCaa                        >  2:727790/1‑91 (MQ=255)                                                               aaGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGc                 >  1:712928/1‑91 (MQ=255)                                                               aaGGCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGc                 >  1:521401/1‑91 (MQ=255)                                                                  gCCGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAgg              >  2:530027/1‑91 (MQ=255)                                                                    cGTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCg            <  2:221997/91‑1 (MQ=255)                                                                     gTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCCTACTCGGGCCGCAAATTGGGCAGGCGa           >  2:60988/1‑91 (MQ=255)                                                                     gTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCg                           <  1:287572/75‑1 (MQ=255)                                                                     gTCGCAGGTCGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCg                           >  2:287572/1‑75 (MQ=255)                                                                              cGGGCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCgg  <  1:576340/91‑1 (MQ=255)                                                                               gggCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCt                                                  >  1:827165/1‑42 (MQ=255)                                                                               gggCAGG‑CCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCt                                                  <  2:827165/42‑1 (MQ=255)                                                                                      |                                                                                    CCAGCCAGCGACAGTTCCGGATTCTGGATCACTCGATCAGGATCGAAATATTGCTGTACGCCAAGGCCGTCGCAGGTCGGGCAGGCCCCCGCCGGGTTGTTAAACGAAAACAGTCGCGGCTCCAGTTCACGCATACTGTAGCCGCAAATTGGGCAGGCGAAGTTGGCGG  >  NZ_CP009273/4262889‑4263057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑