Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	475,405	Δ1 bp	intergenic (‑31/+141)	maa ← / ← hha	maltose O‑acetyltransferase/HHA domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	475,404	0	A	.	100.0%	156.9 / NA	35	intergenic (‑30/+142)	maa/hha	maltose O‑acetyltransferase/HHA domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (16/19);  total (16/19)

CGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGCAACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAGGGTATGTCTTCAT  >  NZ_CP009273/475315‑475484                                                                                          |                                                                                 cGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGCac                                                                                 >  2:529623/1‑90 (MQ=255) cGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGCac                                                                                 <  1:529623/91‑2 (MQ=255)   tctcATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGCacac                                                                               <  2:161867/91‑4 (MQ=255)     tcATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAg                                                                            >  2:53525/1‑91 (MQ=255)       aTCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTa                                                                          <  1:30183/91‑1 (MQ=255)              gAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGaa                                                                   >  2:81360/1‑91 (MQ=255)              gAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGaa                                                                   >  2:378901/1‑91 (MQ=255)              gAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGaa                                                                   >  1:180194/1‑91 (MQ=255)                gCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGaaaa                                                                 >  2:796722/1‑91 (MQ=255)                           cACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAaga                                                      >  1:123073/1‑91 (MQ=255)                             ccAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAgaga                                                    >  2:705915/1‑91 (MQ=255)                              cAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGaa                                                   <  2:326698/91‑1 (MQ=255)                                gCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGCacac                                                                               <  2:6193/62‑4 (MQ=255)                                gCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGCacac                                                                               >  1:6193/1‑59 (MQ=255)                                   atcatcTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTc                                              <  1:484847/91‑1 (MQ=255)                                        cttttcttttTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCAtt                                         <  1:865787/91‑1 (MQ=255)                                         tttcctttttcgTTGCTCATTACTGACCTCCGGTTGTTGAAAATGCGGC‑ACACAGTAAAACGAAAAGTCATTGAAGAGAAC‑TTTCCATTAc                                       <  2:487268/91‑1 (MQ=255)                                             ctttttctGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATc                                    <  1:313216/91‑1 (MQ=255)                                             ctttttctGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATc                                    <  2:51867/91‑1 (MQ=255)                                                 ttctGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCtgtg                                <  1:833118/91‑1 (MQ=255)                                                     gtgCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGAt                            >  1:155858/1‑91 (MQ=255)                                                      tgCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATa                           <  1:770409/91‑1 (MQ=255)                                                      tgCTCATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATa                           <  1:214794/91‑1 (MQ=255)                                                          cATTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGa                       <  2:867444/91‑1 (MQ=255)                                                           aTTACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGAt                      <  1:155478/91‑1 (MQ=255)                                                            ttACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCtg                                  >  2:250040/1‑78 (MQ=255)                                                            ttACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCtg                                  <  1:250040/78‑1 (MQ=255)                                                            ttACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGaac                     <  1:460108/91‑3 (MQ=255)                                                            ttACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATc                     <  2:28303/91‑1 (MQ=255)                                                             tACTGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATca                    <  2:178906/91‑1 (MQ=255)                                                                tGACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACAt                 <  1:81360/91‑1 (MQ=255)                                                                 gACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATa                >  1:94610/1‑91 (MQ=255)                                                                 gACCTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATa                >  1:57647/1‑91 (MQ=255)                                                                   ccTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAgg              <  2:180194/91‑1 (MQ=255)                                                                    cTCCGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAggg             >  1:844536/1‑91 (MQ=255)                                                                      ccGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAGGGTa           >  2:309929/1‑91 (MQ=255)                                                                      ccGGTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAGGGTa           >  2:404567/1‑91 (MQ=255)                                                                        ggTTGCTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAGGGTATg         >  1:105353/1‑91 (MQ=255)                                                                             cTGAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAGGGTATGTCTTc    >  1:702846/1‑91 (MQ=255)                                                                               gAAAATGCGGC‑ACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAGGGTATGTCTTCAt  <  2:510033/91‑1 (MQ=255)                                                                                          |                                                                                 CGTCTCATCTGCCGAGCGATACAACTCACCAGCAATCATCTTTTCTTTTTCTGTGCTCATTACTGACCTCCGGTTGCTGAAAATGCGGCAACACAGTAAGACGAAAAGTCAGTGAAGAGAACGTTCCCATTACATCTGTGTGATAAAGATCACATAGGGTATGTCTTCAT  >  NZ_CP009273/475315‑475484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑