Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	481,730	Δ1 bp	coding (514/648 nt)	acrR →	multidrug efflux transporter transcriptional repressor AcrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	481,728	0	T	.	100.0%	131.7 / NA	30	coding (512/648 nt)	acrR	multidrug efflux transporter transcriptional repressor AcrR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (16/14);  total (16/14)

AACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTAC  >  NZ_CP009273/481645‑481811                                                                                    |                                                                                    aaCGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGcc                                                                             >  2:608967/1‑91 (MQ=255) aaCGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGcc                                                                             >  2:401852/1‑91 (MQ=255) aaCGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGcc                                                                             >  2:498963/1‑91 (MQ=255)  aCGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCt                                                                            <  2:126191/91‑1 (MQ=255)               gTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTgg                                                               >  1:534268/1‑91 (MQ=255)                         gAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTg                                                        <  2:270335/88‑1 (MQ=255)                         gAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTg                                                        >  1:270335/1‑88 (MQ=255)                         gAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGccc                                                     >  2:537958/1‑91 (MQ=255)                         gAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGccc                                                     <  2:600548/91‑1 (MQ=255)                            aaTGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGc                                                  <  2:423377/91‑1 (MQ=255)                            aaTGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGc                                                  <  1:368329/91‑1 (MQ=255)                            aaTGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGc                                                  <  2:744435/91‑1 (MQ=255)                            aaTGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGc                                                  <  2:205145/91‑1 (MQ=255)                              tGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCaa                                                <  1:792469/91‑1 (MQ=255)                                ttGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGACACCTGGCTCTTTGCCCCGccctc                                              >  1:160847/1‑87 (MQ=255)                                 tGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCt                                             >  1:106800/1‑91 (MQ=255)                                                   cgcgTCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAGAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAaagaa                           >  1:814238/1‑91 (MQ=255)                                                       tCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGccc                       <  2:498361/91‑1 (MQ=255)                                                       tCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGccc                       <  1:344151/91‑1 (MQ=255)                                                       tCGCGCAGCAATTATTATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGccc                       <  1:216550/91‑1 (MQ=255)                                                                 attattATGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGt             >  1:790451/1‑91 (MQ=255)                                                                       atGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCAt       <  1:537958/91‑1 (MQ=255)                                                                        tGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATc      >  1:854613/1‑91 (MQ=255)                                                                        tGCGCGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATc      >  2:820779/1‑91 (MQ=255)                                                                         gcgcGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCt     >  1:768327/1‑91 (MQ=255)                                                                         gcgcGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCt     <  2:51986/91‑1 (MQ=255)                                                                         gcgcGGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCt     >  1:467673/1‑91 (MQ=255)                                                                           gcgGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTa   >  1:803737/1‑91 (MQ=255)                                                                            cgGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTAc  >  1:788763/1‑91 (MQ=255)                                                                            cgGCTATA‑TTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTAc  <  1:700640/91‑1 (MQ=255)                                                                                    |                                                                                    AACGTTAAAACATTGTATTGAAGCGAAAATGTTGCCTGCGGATTTAATGACGCGTCGCGCAGCAATTATTATGCGCGGCTATATTTCCGGCCTGATGGAAAACTGGCTCTTTGCCCCGCAATCTTTTGATCTTAAAAAAGAAGCCCGCGATTACGTTGCCATCTTAC  >  NZ_CP009273/481645‑481811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑