Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	945,384	(T)5→4	coding (261/591 nt)	ycaK →	NAD(P)H oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	945,380	0	T	.	100.0%	219.6 / NA	48	coding (257/591 nt)	ycaK	NAD(P)H oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (20/28);  total (20/28)

AGACTGGAAGAATATGGATAAACGTTATTCTCCAGAGGTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGGTTTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATG  >  NZ_CP009273/945294‑945466                                                                                       |                                                                                       aGACTGGAAGAATATGGATAAACGTTATTCTCCAGAGGTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGGttttc                                                                                    <  2:84025/91‑2 (MQ=255)  gACTGGAAGAATATGGATAAACGTTATTCTCCAGAGGTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGGttttcc                                                                                   >  2:628231/1‑89 (MQ=255)  gACTGGAAGAATATGGATAAACGTTATTCTCCAGAGGTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGGttttcc                                                                                   <  2:268855/91‑3 (MQ=255)       gaagaaTATGGATAAACGTTATTCTCCAGAGGTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCctctct                                                                             <  2:152466/91‑1 (MQ=255)            atatGGATAAACGTTATTCTCCAGAGGTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCtggtgg                                                                        >  1:622478/1‑91 (MQ=255)                ggATAAACGTTATTCTCCAGAGGTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACa                                                                    <  1:190626/91‑1 (MQ=255)                ggATAAACGTTATTCTCCAGAGGTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACa                                                                    <  2:396678/91‑1 (MQ=255)                 gATAAACGTTATTCTCCAGAGGTTCATTAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAg                                                                   >  2:232790/1‑91 (MQ=255)                          tATTCTCCAGAGGTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGc                                                          >  1:225192/1‑91 (MQ=255)                          tATTCTCCAGAGGTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGc                                                          <  2:364882/91‑1 (MQ=255)                           aTTCTCCAGAGGTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCa                                                         <  2:559161/91‑1 (MQ=255)                                ccAGAGGTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCt                                                    >  1:87196/1‑91 (MQ=255)                                  agagGTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTaa                                                  <  2:140097/91‑1 (MQ=255)                                    agGTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTaaaa                                                <  2:357129/91‑1 (MQ=255)                                     ggTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAg                                               >  2:203566/1‑91 (MQ=255)                                      gTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAgg                                              <  1:5041/91‑1 (MQ=255)                                      gTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAgg                                              <  1:203566/91‑1 (MQ=255)                                      gTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAgg                                              <  1:695814/91‑1 (MQ=255)                                          atcaGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGatat                                          >  1:573771/1‑91 (MQ=255)                                                tttATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGAc                                    <  2:225192/91‑1 (MQ=255)                                                tttATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGAc                                    <  1:292472/91‑1 (MQ=255)                                                  tATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACag                                  <  2:89878/91‑1 (MQ=255)                                                  tATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACag                                  <  1:749607/91‑1 (MQ=255)                                                  tATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACag                                  <  1:616340/91‑1 (MQ=255)                                                  tATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACag                                  <  2:635588/91‑1 (MQ=255)                                                  tATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACag                                  <  1:628231/91‑1 (MQ=255)                                                  tATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACag                                  <  1:38371/91‑1 (MQ=255)                                                    ttCAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACagag                                <  1:325933/91‑1 (MQ=255)                                                      cAGAGCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTa                              <  2:426562/91‑1 (MQ=255)                                                        gagCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAgg                                              <  1:729055/73‑1 (MQ=255)                                                        gagCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAgg                                              >  2:729055/1‑73 (MQ=255)                                                        gagCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATg                            >  1:364810/1‑91 (MQ=255)                                                        gagCTGCTTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATg                            >  1:458422/1‑91 (MQ=255)                                                             gctTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTaaaa                                                >  2:268690/1‑66 (MQ=255)                                                             gctTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTaaaa                                                <  1:268690/66‑1 (MQ=255)                                                             gctTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGaata                       >  1:493712/1‑91 (MQ=255)                                                             gctTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGaata                       >  1:711383/1‑91 (MQ=255)                                                             gctTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGaata                       >  1:219793/1‑91 (MQ=255)                                                             gctTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGaata                       >  1:253545/1‑91 (MQ=255)                                                             gctTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGaata                       >  2:396842/1‑91 (MQ=255)                                                             gctTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGaata                       >  1:620367/1‑91 (MQ=255)                                                             gctTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGaata                       >  1:9785/1‑91 (MQ=255)                                                             gctTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGaata                       >  1:625263/1‑91 (MQ=255)                                                             gctTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAAGGCTAAAAGGATATATTGACAGAGTATGGaata                       >  1:837723/1‑91 (MQ=255)                                                              ctTGAACATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGaataa                      <  1:90590/91‑1 (MQ=255)                                                                   aCATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGggc                 <  2:87196/91‑1 (MQ=255)                                                                   aCATGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGATACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGggc                 <  1:864582/91‑1 (MQ=255)                                                                      tGACACGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGggctgg              <  2:81483/91‑1 (MQ=255)                                                                        acacGTTAGTGGTGG‑TTTTGCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGggctggct            <  2:588818/91‑1 (MQ=255)                                                                        acacGTTAGTGGTGG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGggctggct            <  2:321762/91‑1 (MQ=255)                                                                                gtggtgG‑TTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGgaga    >  2:293752/1‑91 (MQ=255)                                                                                  ggtgg‑tTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATg  >  2:490414/1‑91 (MQ=255)                                                                                  ggtgg‑tTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATg  >  1:15102/1‑91 (MQ=255)                                                                                       |                                                                                       AGACTGGAAGAATATGGATAAACGTTATTCTCCAGAGGTTCATCAGCTTTATTCAGAGCTGCTTGAACATGACACGTTAGTGGTGGTTTTTCCTCTCTGGTGGTACAGCTTCCCGGCAATGCTAAAAGGATATATTGACAGAGTATGGAATAATGGGCTGGCTTATGGAGATG  >  NZ_CP009273/945294‑945466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑