Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,246,085	(C)5→4	coding (210/1071 nt)	dhaK ←	dihydroxyacetone kinase subunit DhaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,246,081	0	C	.	100.0%	128.0 / NA	29	coding (214/1071 nt)	dhaK	dihydroxyacetone kinase subunit DhaK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (12/17);  total (12/17)

GTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTT  >  NZ_CP009273/1245997‑1246162                                                                                     |                                                                                  gTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGccccgaa                                                                             >  1:390962/1‑88 (MQ=255)   acacCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAg                                                                          >  1:459741/1‑91 (MQ=255)     acCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCa                                                                        <  2:33727/91‑1 (MQ=255)        ttCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATcc                                                                     >  1:12805/1‑91 (MQ=255)        ttCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATcc                                                                     >  2:270897/1‑91 (MQ=255)        ttCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATcc                                                                     >  2:521661/1‑91 (MQ=255)        ttCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATcc                                                                     >  2:641371/1‑91 (MQ=255)         tCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATccc                                                                    >  2:281198/1‑91 (MQ=255)             cgccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGa                                                                <  1:270897/91‑1 (MQ=255)              gccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGAc                                                               <  1:540889/91‑1 (MQ=255)              gccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGAc                                                               <  1:675256/91‑1 (MQ=255)               ccATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGAcc                                                              <  2:853951/91‑1 (MQ=255)                cATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCg                                                             <  1:521661/91‑1 (MQ=255)                cATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCg                                                             <  2:822834/91‑1 (MQ=255)                  tCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCGat                                                           <  1:236365/91‑1 (MQ=255)                        tGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCGATATAAcc                                                     >  2:672390/1‑91 (MQ=255)                           aTGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCGATATAACcaca                                                  >  2:316633/1‑91 (MQ=255)                             ggCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGt                                                >  1:311524/1‑91 (MQ=255)                              gcgcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTg                                               >  2:565503/1‑91 (MQ=255)                                gcATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCa                                             >  1:185948/1‑91 (MQ=255)                                     cAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGc                                        <  1:214000/91‑1 (MQ=255)                                       aaGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCGATATAACCACGGTGCATCGGCTc                                      <  1:403969/91‑1 (MQ=255)                                            tttATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTc                                 <  2:21510/91‑1 (MQ=255)                                                   ggCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgcc                          <  1:593458/91‑1 (MQ=255)                                                   ggCGTCGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTgcc                          <  1:575307/91‑1 (MQ=255)                                                        cGGTGAGGTGAAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCAc                     <  2:201858/91‑1 (MQ=255)                                                           tgaggtgaAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCg                  <  1:285202/91‑1 (MQ=255)                                                                 gaAAATTTCGCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGAcagca            <  2:391152/91‑1 (MQ=255)                                                                          gCCCGGACAGG‑CCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACtt   <  1:91486/91‑1 (MQ=255)                                                                           cccGGACAGG‑CCCCGAAAGCATCCCCTGGCCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGCCAGCAGGGCGCCttt  >  2:606983/1‑91 (MQ=255)                                                                                     |                                                                                  GTACACCTTCGCCGCCATCAACTTGCATGGCGCATTCAAAGATTTTATCGGGCGTCGGTGAGGTGAAAATTTCGCCCGGACAGGCCCCCGAAAGCATCCCCTGACCGATATAACCACAGTGCATCGGCTCGTGTCCGCTGCCGCCACCCGACAGCAGGGCGACTTT  >  NZ_CP009273/1245997‑1246162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑