Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,659,452	(T)7→6	intergenic (+75/‑84)	dmsD → / → clcB	Tat proofreading chaperone DmsD/voltage‑gated ClC‑type chloride channel ClcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,659,446	0	T	.	100.0%	137.8 / NA	31	intergenic (+69/‑90)	dmsD/clcB	Tat proofreading chaperone DmsD/voltage‑gated ClC‑type chloride channel ClcB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (22/9);  total (22/9)

GGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTG  >  NZ_CP009273/1659356‑1659532                                                                                           |                                                                                       ggTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGt                                                                                        <  2:716997/91‑1 (MQ=255) ggTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGt                                                                                        <  2:222608/91‑1 (MQ=255)       aCCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGtttttta                                                                                  <  2:98649/91‑2 (MQ=255)         cGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGttttttatc                                                                                <  2:144908/91‑4 (MQ=255)                       gATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAgcacgca                                                                 >  1:677517/1‑91 (MQ=255)                                 atatTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGc                                                       >  2:632041/1‑91 (MQ=255)                                 atatTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGc                                                       >  2:360451/1‑91 (MQ=255)                                      gCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTg                                                  >  1:208627/1‑91 (MQ=255)                                      gCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTg                                                  >  2:641484/1‑91 (MQ=255)                                      gCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTg                                                  >  1:55878/1‑91 (MQ=255)                                      gCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTg                                                  >  1:779291/1‑91 (MQ=255)                                      gCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTg                                                  >  2:414547/1‑91 (MQ=255)                                      gCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTg                                                  >  2:349451/1‑91 (MQ=255)                                                  cGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGcc                                      <  1:65597/91‑1 (MQ=255)                                                       aCGCGCAATCCGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGGCTCTTTTGCCGCaaa                                 >  2:680164/1‑91 (MQ=255)                                                                ccGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGcc                        <  1:414547/91‑1 (MQ=255)                                                                ccGGCAATAATAGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGcc                        <  2:55878/91‑1 (MQ=255)                                                                        aataGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGcc                        >  1:311136/1‑83 (MQ=255)                                                                        aataGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGcc                        <  2:311136/83‑1 (MQ=255)                                                                        aataGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTc                >  2:527599/1‑91 (MQ=255)                                                                          taGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCAt              <  2:224052/91‑1 (MQ=255)                                                                          taGGTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCAt              <  2:600532/91‑1 (MQ=255)                                                                             gTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGc           >  2:519937/1‑91 (MQ=255)                                                                             gTTACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGc           >  1:843256/1‑91 (MQ=255)                                                                              ttACAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGcc          >  2:195638/1‑91 (MQ=255)                                                                                aCAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCa        >  2:8928/1‑91 (MQ=255)                                                                                 cAGTGTCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCAt       >  1:203790/1‑91 (MQ=255)                                                                                    tgtCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTc    >  2:37907/1‑91 (MQ=255)                                                                                    tgtCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTc    <  1:527599/91‑1 (MQ=255)                                                                                    tgtCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTc    <  1:349451/91‑1 (MQ=255)                                                                                     gtCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCt   >  2:176991/1‑91 (MQ=255)                                                                                     gtCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCt   >  1:628100/1‑91 (MQ=255)                                                                                     gtCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCt   <  2:779291/91‑1 (MQ=255)                                                                                      tCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTg  >  1:147104/1‑91 (MQ=255)                                                                                      tCACG‑TTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTg  >  1:110284/1‑91 (MQ=255)                                                                                           |                                                                                       GGTTAAACCGCTGTTTCGATAGGATCACCGTAATATTGCCGGATGGTGACGATTACGCGCAATCCGGCAATAATAGGTTACAGTGTCACGTTTTTTTATCTCTTAAAGCACGCACTGCTTTTGCGGCTGGCCTCTTTTGCCGCAAAATAGTCGCCCGTGTTTCATTGCCCATTTCTG  >  NZ_CP009273/1659356‑1659532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑