| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,614,266 | (A)8→9 | intergenic (‑35/‑290) | upp ← / → purM | uracil phosphoribosyltransferase/phosphoribosylformylglycinamidine cyclo‑ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,614,258 | 1 | . | A | 95.5% | 74.9 / ‑3.7 | 22 | intergenic (‑27/‑298) | upp/purM | uracil phosphoribosyltransferase/phosphoribosylformylglycinamidine cyclo‑ligase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (11/10); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAA‑AATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTT > NZ_CP009273/2614168‑2614340 | cTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCcac > 1:595858/1‑91 (MQ=255) aCGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑aaaaaa‑a > 1:158650/1‑91 (MQ=255) aGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA‑AATCGAc < 2:666242/91‑1 (MQ=255) aGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA‑AATCGAc < 1:555127/91‑1 (MQ=255) gTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCCCCTTTGAGGGGCAGCCACAAAAAAA‑AATCGACg < 1:737055/91‑1 (MQ=255) tgtgTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA‑AATCGACGGATTATAcc < 1:283857/91‑1 (MQ=255) gtgtTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA‑AATCGACGGATTATAcct > 1:108246/1‑91 (MQ=255) gtgtTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA‑AATCGACGGATTATAcct > 1:281442/1‑91 (MQ=255) gtgtTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA‑AATCGACGGATTATAcct > 2:317819/1‑91 (MQ=255) gtgtTTGACGAGGGGGGGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAACAATCGACGGATTATAcct > 2:311581/1‑91 (MQ=255) gTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA‑AATCGACGGATTATACCTCCTTTCTTCaa > 1:484904/1‑91 (MQ=255) ggTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA‑AATCGACGGATTATACCTCCTTTCTTCAAggc < 2:441106/91‑1 (MQ=255) tGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA‑AATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAat > 1:95630/1‑91 (MQ=255) aCTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA‑AATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAatat < 1:173103/91‑1 (MQ=255) tCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA‑AATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCt > 1:111212/1‑91 (MQ=255) tCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA‑AATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCt > 2:154730/1‑91 (MQ=255) aCGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCACAAAAAAA‑AATCGACGGATTATCCCTCCTTTCTTCAAGGCGGCAATATTCtttt > 1:569979/1‑91 (MQ=255) tttCTCCTTTGAGGGGCAGCCACAAAAAAA‑AATCGACGGATTATACCTCCtt < 1:675903/52‑1 (MQ=255) tttCTCCTTTGAGGGGCAGCCACAAAAAAA‑AATCGACGGATTATACCTCCtt > 2:675903/1‑52 (MQ=255) tcttctTTGAGGGGCAGCCACAAAAAAA‑AATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCaaa < 2:290839/91‑1 (MQ=255) tctcCTTTGAGGGGCAGCCACAAAAAAA‑AATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTGGTTGACTTTAGTCaaa > 1:780609/1‑91 (MQ=255) gAGGGGCAGCCACAAAAAAA‑AATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAAc < 1:311131/91‑1 (MQ=255) gCAGCCACAAAAAAA‑AATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGttt < 1:846612/91‑1 (MQ=255) gCAGCCACAAAAAAA‑AATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGttt > 2:846612/1‑91 (MQ=255) | CTTGCTCACGCATCAGTCCCAGCTTGTGTTTGACGAGTGGGTGTTTGACTTCCACGATCTTCATACTCTTTCTCCTTTGAGGGGCAGCCAC‑AAAAAA‑AATCGACGGATTATACCTCCTTTCTTCAAGGCGGCAATATTCTTTTCGTTGACTTTAGTCAAAATGATAACGGTTT > NZ_CP009273/2614168‑2614340 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |