Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,292,953	+C	coding (322/444 nt)	yhbP ←	YhbP family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,292,952	1	.	C	100.0%	39.4 / NA	12	A108G (GCC→GGC)	yhbP	YhbP family protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (4/8);  total (4/8)

AATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGG‑CAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACT  >  NZ_CP009273/3292864‑3293033                                                                                          |                                                                                  aaTCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGG‑cc                                                                                 >  2:357269/1‑90 (MQ=255)          tttACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGaaa                                                                         <  1:684456/91‑1 (MQ=255)                aaaGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGcgacg                                                                   >  1:336650/1‑91 (MQ=255)                aaaGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGcgacg                                                                   <  2:250568/91‑1 (MQ=255)                                                  tCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCa                                 <  1:357269/91‑1 (MQ=255)                                                  tCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCa                                 <  2:827872/91‑1 (MQ=255)                                                       ccGGATTTCCCACACCGGGGCCGACAGCATTCGGGCCAACCGGAAAGCGACGAGGGGACGCCGTGCGCGCGAGGTCGCTTTCCTCACCTTc                            <  2:140302/91‑1 (MQ=255)                                                                    acCGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAtc               <  2:112355/91‑1 (MQ=255)                                                                      cGGGGCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGAtctc             >  2:93592/1‑91 (MQ=255)                                                                        gggCCGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCAc           >  2:378308/1‑91 (MQ=255)                                                                           ccGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCtt        <  2:167380/91‑1 (MQ=255)                                                                           ccGACAGCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCtt        <  2:567279/91‑1 (MQ=255)                                                                                 gCATTCTGGCCAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACt  >  1:208493/1‑91 (MQ=255)                                                                                          |                                                                                  AATCATTTTTTTACCAAAGCCCAGCGTGTTGTCGGTGAATTTGATTTCATCGAGCCGGATTTCCCACACCGGGGCCGACAGCATTCTGG‑CAACCGGAAAGCGACGATTGTACGCCTTGCGCGCGAGGTCGCTTTCCTCACCTTCCAGCCTGCGGATCTCACCTTTAAACT  >  NZ_CP009273/3292864‑3293033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑