BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	322,719	0	T	C	35.8%	118.2 / 115.7	109	G80G (GGA→GGG)	betA	choline dehydrogenase
Reads supporting (aligned to +/- strand): ref base T (31/39); new base C (16/23); total (47/62)
Fisher's exact test for biased strand distribution p-value = 8.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 111 total aligned reads displayed.

CTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTACCCTGTAGCGGGAATGCCAG  >  NZ_CP009273/322631‑322808

cTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGccc                                                                                         <  1:382578/91‑1 (MQ=255)

 ttGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCg                                                                                        <  1:169822/91‑1 (MQ=255)

   gcgcCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCa                                                                                      <  2:9806/91‑1 (MQ=255)

     gcCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACt                                                                                    >  1:551648/1‑91 (MQ=255)

     gcCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACt                                                                                    <  2:164246/91‑1 (MQ=255)

      cccAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTc                                                                                   <  1:69577/91‑1 (MQ=255)

         aGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCAt                                                                                <  1:687675/91‑1 (MQ=255)

            tATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACCATCCACCCAGACCTTTACCGCGTCCGCACTCCATgcg                                                                             <  1:336205/91‑1 (MQ=255)

              tCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATgcggc                                                                           <  1:117529/91‑1 (MQ=255)

              tCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATgcggc                                                                           <  2:480744/91‑1 (MQ=255)

                 agaTCCAGCGCATTGCCACGGATGTAGCACATTCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGt                                                                        <  2:644095/91‑1 (MQ=255)

                  gaTCCAGCGCATTGCCACGGATGTAGCACATTCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGtt                                                                       <  2:28977/91‑1 (MQ=255)

                  gaTCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTGCCGCGCCCGCACTCCATGCGGCGGtt                                                                       <  2:524963/91‑1 (MQ=255)

                  gaTCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGtt                                                                       >  2:428615/1‑91 (MQ=255)

                  gaTCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGtt                                                                       >  2:570449/1‑91 (MQ=255)

                  gaTCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGtt                                                                       <  2:436108/91‑1 (MQ=255)

                  gaTCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGtt                                                                       >  1:683297/1‑91 (MQ=255)

                  gaTCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGtt                                                                       <  2:626121/91‑1 (MQ=255)

                  gaTCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGtt                                                                       <  2:446072/91‑1 (MQ=255)

                    tCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTAt                                                                     <  1:683461/91‑1 (MQ=255)

                    tCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTAt                                                                     >  1:490055/1‑91 (MQ=255)

                    tCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGt                                                                        >  1:659674/1‑88 (MQ=255)

                    tCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGt                                                                        <  2:659674/88‑1 (MQ=255)

                    tCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTAt                                                                     >  1:650055/1‑91 (MQ=255)

                    tCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTAt                                                                     >  1:811111/1‑91 (MQ=255)

                        gcgcATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCAt                                                                 >  2:173678/1‑91 (MQ=255)

                          gcATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATaa                                                               <  1:361635/91‑1 (MQ=255)

                            aTTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAAc                                                             <  2:4962/91‑1 (MQ=255)

                            aTTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAAc                                                             <  1:792877/91‑1 (MQ=255)

                             ttGCCACGGATGTAGCACATTCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACg                                                            <  2:538419/91‑1 (MQ=255)

                             ttGCCACGGATGTAGCACATTCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACg                                                            <  2:137063/91‑1 (MQ=255)

                             ttGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACg                                                            >  1:834276/1‑91 (MQ=255)

                                       tGTAGCCCATTCCGTTGATCAGCGCCGATCCCCCCAGCCCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTc                                                  <  2:247239/91‑1 (MQ=255)

                                       tGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTc                                                  >  2:214983/1‑91 (MQ=255)

                                       tGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTc                                                  <  1:660721/91‑1 (MQ=255)

                                         tAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACg                                                            >  1:55227/1‑79 (MQ=255)

                                         tAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACg                                                            <  2:55227/79‑1 (MQ=255)

                                         tAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCg                                                >  1:98080/1‑91 (MQ=255)

                                         tAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCg                                                >  1:288629/1‑91 (MQ=255)

                                         tAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCg                                                >  2:78528/1‑91 (MQ=255)

                                         tAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCg                                                >  1:412924/1‑91 (MQ=255)

                                             acaTGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTc                                            >  1:13748/1‑91 (MQ=255)

                                               attccGTTGATCAGCGACGATCCACCGAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCAt                                          <  2:524082/88‑1 (MQ=255)

                                               aTGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCAt                                          >  2:731995/1‑91 (MQ=255)

                                               aTGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCAt                                          >  2:366237/1‑91 (MQ=255)

                                               aTGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCAt                                          >  2:817419/1‑91 (MQ=255)

                                               aTGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCAt                                          >  2:618857/1‑91 (MQ=255)

                                               aTGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCAt                                          >  1:584891/1‑91 (MQ=255)

                                               aTGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCAt                                          <  1:570449/91‑1 (MQ=255)

                                               aTGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGtt                                                                       >  1:408400/1‑62 (MQ=255)

                                               aTGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCAt                                          >  1:739572/1‑91 (MQ=255)

                                               aTGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCAt                                          >  1:38614/1‑91 (MQ=255)

                                               aTGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCAt                                          >  2:214647/1‑91 (MQ=255)

                                                tGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATa                                         >  1:602771/1‑91 (MQ=255)

                                                tGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATa                                         >  2:165098/1‑91 (MQ=255)

                                                tGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATa                                         <  1:68841/91‑1 (MQ=255)

                                                  ccGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCACGTTCCGTTTCATAgg                                       >  2:240528/1‑91 (MQ=255)

                                                     ttGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGccc                                    >  1:804363/1‑91 (MQ=255)

                                                     ttGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGccc                                    <  2:152400/91‑1 (MQ=255)

                                                     ttGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGccc                                    >  2:470733/1‑91 (MQ=255)

                                                     ttGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGccc                                    >  1:171855/1‑91 (MQ=255)

                                                     ttGATCAGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGccc                                    <  1:185719/91‑1 (MQ=255)

                                                         tCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGtt                                                                       >  1:109726/1‑52 (MQ=255)

                                                         tCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGtt                                                                       <  2:109726/52‑1 (MQ=255)

                                                           aGCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGt                              <  1:820067/91‑1 (MQ=255)

                                                            gCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAg                                        >  1:429563/1‑80 (MQ=255)

                                                            gCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAg                                        <  2:429563/80‑1 (MQ=255)

                                                            gCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTa                             >  1:37317/1‑91 (MQ=255)

                                                            gCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTa                             <  2:388592/91‑1 (MQ=255)

                                                            gCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTa                             <  1:618857/91‑1 (MQ=255)

                                                            gCGACGATCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTa                             <  1:206398/91‑1 (MQ=255)

                                                                 gaTCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGtt                        <  2:171855/91‑1 (MQ=255)

                                                                 gaTCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGtt                        >  1:349268/1‑91 (MQ=255)

                                                                 gaTCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGtt                        <  1:817419/91‑1 (MQ=255)

                                                                 gaTCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGtt                        >  1:105469/1‑91 (MQ=255)

                                                                 gaTCCACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGtt                        <  1:59856/91‑1 (MQ=255)

                                                                     cACCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTAcc                    <  1:214647/91‑1 (MQ=255)

                                                                      aCCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCTGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTAccc                   <  1:11883/91‑1 (MQ=255)

                                                                      aCCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTAccc                   <  2:28709/91‑1 (MQ=255)

                                                                      aCCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTAccc                   <  1:366237/91‑1 (MQ=255)

                                                                      aCCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTAccc                   <  2:811111/91‑1 (MQ=255)

                                                                      aCCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTAccc                   <  1:173678/91‑1 (MQ=255)

                                                                      aCCCAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTAccc                   >  1:407511/1‑91 (MQ=255)

                                                                       cccAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTACCCt                  <  1:179867/91‑1 (MQ=255)

                                                                        ccAGACCTTTACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTACCCTg                 <  1:202570/91‑1 (MQ=255)

                                                                          aGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTACCCTGTa               <  1:274517/91‑1 (MQ=255)

                                                                           gACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTACCCTGTAg              <  1:606888/91‑1 (MQ=255)

                                                                           gACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTACCCTGTAg              >  2:868967/1‑91 (MQ=255)

                                                                           gACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTACCCTGTAg              >  2:870989/1‑91 (MQ=255)

                                                                                ttcccGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTACCCTGTAGCGGGa         <  2:374764/88‑1 (MQ=255)

                                                                                ttACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGGGTTTGCCCTGTAGCGGGa         <  2:95005/91‑1 (MQ=255)

                                                                                ttACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAAGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTACCCTGTAGCGGGa         >  1:306985/1‑91 (MQ=255)

                                                                                ttACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGccc                                    <  2:690631/64‑1 (MQ=255)

                                                                                ttACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGccc                                    >  1:690631/1‑64 (MQ=255)

                                                                                ttACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTACCCTGTAGCGGGa         <  2:146438/91‑1 (MQ=255)

                                                                                ttACCGCGCCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTACCCTGTAGCGGGa         >  1:867907/1‑91 (MQ=255)

                                                                                  aCCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTACCCTGTAGCGGGAAt       <  2:126760/91‑1 (MQ=255)

                                                                                     gcgcCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTACCCTGTAGCGGGAATGcc    >  2:143237/1‑91 (MQ=255)

                                                                                     gcgTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTACCCTGTAGCGGGAATGcc    >  2:539274/1‑91 (MQ=255)

                                                                                       gTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTACCCTGTAGCGGGAATGCCAg  <  2:804363/91‑1 (MQ=255)

CTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCATAGGCCCAGTTGTAGCGTTTACCCTGTAGCGGGAATGCCAG  >  NZ_CP009273/322631‑322808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`