| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 361,532 | 0 | A | C | 51.0% | ‑4.3 / 93.9 | 51 | L71R (CTG→CGG) | lacI | DNA‑binding transcriptional repressor LacI |
| Reads supporting (aligned to +/- strand): ref base A (13/12); new base C (14/12); total (27/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCG > NZ_CP009273/361443‑361622 | gTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAg < 2:249477/91‑1 (MQ=255) cTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACt < 2:286377/91‑1 (MQ=255) cTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACt > 2:351130/1‑91 (MQ=255) cTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACt > 1:530706/1‑91 (MQ=255) cTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACt < 1:379639/91‑1 (MQ=255) aCCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTgg > 1:437574/1‑91 (MQ=255) cATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAg < 2:839417/91‑1 (MQ=255) aTCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAgg > 2:43432/1‑91 (MQ=255) aTCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAgg > 2:658833/1‑91 (MQ=255) cGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTg > 2:184989/1‑91 (MQ=255) cGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTg > 1:667888/1‑91 (MQ=255) cGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTg > 1:43832/1‑91 (MQ=255) gACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTgg > 2:154120/1‑91 (MQ=255) caccaccacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCGGGGCCAGACTGGAGGTGGCa > 1:702400/1‑91 (MQ=255) caccaccacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCa < 1:184989/91‑1 (MQ=255) ccaccacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAAc > 2:311278/1‑91 (MQ=255) ccaccacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAAc > 2:642100/1‑91 (MQ=255) ccaccacGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAAc < 2:239725/91‑1 (MQ=255) ccaccacGCTGGCACCAAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGGGGCacg > 2:429004/1‑89 (MQ=255) cTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCa < 1:20862/91‑1 (MQ=255) ggCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGc > 2:853425/1‑91 (MQ=255) ggCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGc > 1:735488/1‑91 (MQ=255) ggCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGAAGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGc > 1:665349/1‑91 (MQ=255) cAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGAc > 1:461380/1‑91 (MQ=255) cAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGAc > 2:198544/1‑91 (MQ=255) gTTTATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTg < 2:78314/91‑1 (MQ=255) tGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGAc > 1:116105/1‑58 (MQ=255) tGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGAc < 2:116105/58‑1 (MQ=255) tGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGtt > 2:641389/1‑91 (MQ=255) cGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGcc < 2:176276/91‑1 (MQ=255) gcgcGAGATTTAACCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCg < 2:782096/91‑1 (MQ=255) gcgAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGcc > 1:288219/1‑91 (MQ=255) tAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGttgttg < 1:764173/91‑1 (MQ=255) tAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGttgttg > 1:205170/1‑91 (MQ=255) ccgcGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCAc > 2:713332/1‑91 (MQ=255) gcgACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCAcgc < 2:667888/91‑1 (MQ=255) gcgACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCAcgc < 1:780834/91‑1 (MQ=255) cgACAATTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCAcgcg < 1:336475/91‑1 (MQ=255) gACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCgg < 2:530706/91‑1 (MQ=255) cAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGtt < 1:246630/91‑1 (MQ=255) aaTTTGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTg < 1:30907/91‑1 (MQ=255) aaTTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTg < 1:90496/91‑1 (MQ=255) aaTTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTg < 1:456527/91‑1 (MQ=255) tttGCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTggg < 1:324409/91‑1 (MQ=255) tttGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTggg < 2:412634/91‑1 (MQ=255) tttGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTggg < 1:11042/91‑1 (MQ=255) gCGACGGCGCGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAAt < 1:394760/91‑1 (MQ=255) gcgcGTGCAGGGCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAAtt > 2:523655/1‑91 (MQ=255) gcgcGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAAtt > 1:483989/1‑91 (MQ=255) gggCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGc > 1:660814/1‑91 (MQ=255) ggCCCGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGcc > 1:188677/1‑91 (MQ=255) aGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATcg > 2:612113/1‑91 (MQ=255) aGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATcg > 2:385902/1‑91 (MQ=255) | GTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCG > NZ_CP009273/361443‑361622 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |