| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,398,051 | 0 | G | T | 46.2% | ‑0.3 / 14.1 | 13 | Q258K (CAA→AAA) | abgA | p‑aminobenzoyl‑glutamate hydrolase subunit AbgA |
| Reads supporting (aligned to +/- strand): ref base G (1/6); new base T (6/0); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTTCCTGCCTGCATAACGCCCACGTTTACTCTGGAAGCTCCTTCGCTGTGCGGGGCGATTGCATGCAGTGCAAGAGTGGCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATT > NZ_CP009273/1397968‑1398134 | gCTTCCTGCCTGCATAACGCCCACGTTTACTCTGGAAGCTCCTTCGCTGTGCGGGGCGATTGCATGCAGTGCAAGAGTGGCTTTtggtgtg > 2:373973/1‑89 (MQ=255) cTTCCTGCCTGCATAACGCCCACGTTTACTCTGGAAGCTCCTTCGCTGTGCGGGGCGATTGCATGCAGTGCAAGAGTGGCTTGTGCTgccg < 2:103919/91‑1 (MQ=255) ctgcctgcATAACGCCCACGTTTACTCTGGAAGCTCCTTCGCTGTGCGGGGCGATTGCATGCAGTGCAAGAGTGGCTTGTGCTGCCGCcaa < 1:4571/91‑1 (MQ=255) gcATAACGCCCACGTTTACTCTGGAAGCTCCTTCGCTGTGCGGGGCGATTGCATGCAGTGCAAGAGTGGCTTTTTCTGTCGCCCACAAGGc > 2:18549/1‑91 (MQ=255) cACGTTTACTCTGGAAGCTCCTTCGCTGTGCGGGGCGATTGCATGCAGTGCAAGAGTGGCTTTTGCTTCCGCCAACAAAGGATTTTGAcgg > 2:507128/1‑89 (MQ=255) aCTCTGGAAGCTCCTTCGCTGTGCGGGGCGATTGCATGCAGTGCAAGAGTGGCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTg < 2:224333/91‑1 (MQ=255) tctGGAAGCTCCTTCGCTGTGCGGGGCGATTGCATGCAGTGCAAGAGTGGCTTTTGCTGCCGCCCACAAGGCATTGTGACCGTCTTCTGGt > 2:451520/1‑91 (MQ=255) ggAAGCTCCTTCGCTGTGCGGGGCGATTGCATGCAGTGCAAGAGTGGCTTTTTTTGCCGCCAACAAAGGACTTGGGGCGTCTTCTGGtttt > 2:44028/1‑91 (MQ=255) ggAAGCTCCTTCGCTGTGCGGGGCGATTGCATGCAGTGCAAGAGTGGCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGtttt > 1:660782/1‑91 (MQ=255) cTTCGCTGTGCGGGGCGATTGCATGCAGTGCAAGAGTGGCTTTTGTTGCCGCCCACAAAGCATTGTGACCGTCTTTTTGTTTTTCGCCTGc > 2:418318/1‑91 (MQ=255) tCGCTGTGCGGGGCGATTGCATGCAGTGCAAGAGTGGCTTTTTCTGCCGCCCACAAGGCATTGTGGCCGTCTTCTGGTTTTTTGCCCGCGt > 2:66057/1‑91 (MQ=255) gCTGTGCGGGGCGATTGCATGCAGTGCAAGAGTGGCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGa < 1:66057/91‑1 (MQ=255) gggCGATTGCATGCAGTGCAAGAGTGGCTTTTGTTGCCGCCCCCAAAGGATTTTGACCGGCTTCTTTTTTTTTGCCTGCGTGGGgggcggg > 2:145126/1‑90 (MQ=255) gCGATTGCATGCAGTGCAAGAGTGGCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTAc < 1:834212/91‑1 (MQ=255) gCGATTGCATGCAGTGCAAGAGTGGCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTAc < 1:18549/91‑1 (MQ=255) gTGCAAGAGTGGCTTTTTTTGCCGCCCACAAAGCATTGTGACCGGCTTCTTGTTTTGTGCCCGCGTGGGCGGCGGGACCCGGGGAGTgggg > 2:21877/1‑88 (MQ=255) gTGGCTTTTTTTGCCGCCCACAAAGCATTGTGACCGTCTTCTTGTTTTTTGCCTGCGTGAGCGGCGGGACCGGTGAAGGGGGCGTCaaaat > 2:176780/1‑89 (MQ=255) | GCTTCCTGCCTGCATAACGCCCACGTTTACTCTGGAAGCTCCTTCGCTGTGCGGGGCGATTGCATGCAGTGCAAGAGTGGCTTGTGCTGCCGCCAACAAGGCATTGTGACCGTCTTCTGGTTTTGCGCCTGCGTGAGCGGCGGTACCGGTGAAGTGCGCGTCAAATT > NZ_CP009273/1397968‑1398134 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |