Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	209,618	A→C	A267A (GCA→GCC)	tilS →	tRNA lysidine(34) synthetase TilS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	209,618	0	A	C	100.0%	77.1 / NA	22	A267A (GCA→GCC)	tilS	tRNA lysidine(34) synthetase TilS
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (5/17);  total (5/17)

GGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGC  >  NZ_CP009273/209532‑209705                                                                                       |                                                                                        gggACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGa                                                                                     <  1:54865/91‑1 (MQ=255)                 tgCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGAcgc                                                                     >  1:223473/1‑91 (MQ=255)                   ccAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGt                                                                   <  1:561997/91‑1 (MQ=255)                   ccAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGt                                                                   <  1:811841/91‑1 (MQ=255)                    cAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGtt                                                                  <  2:455477/91‑1 (MQ=255)                    cAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGtt                                                                  <  2:395583/91‑1 (MQ=255)                        gCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTg                                                              <  2:275885/91‑1 (MQ=255)                         cTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGa                                                             <  1:312617/91‑1 (MQ=255)                          tGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAg                                                            >  1:92561/1‑91 (MQ=255)                          tGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAg                                                            >  2:217656/1‑91 (MQ=255)                           ggCGATGAGTGATCCCCGCCGCGGGGCGAGTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTTAgg                                                           <  2:269110/91‑1 (MQ=255)                                 gAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGATGATCTgg                                                     >  2:766152/1‑91 (MQ=255)                                      aTGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGa                                                >  2:814010/1‑91 (MQ=255)                                              cgcgcgGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGtggcgc                                        <  2:328570/91‑1 (MQ=255)                                              cgcgcgGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGtggcgc                                        <  2:570127/91‑1 (MQ=255)                                                     cgATTATCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGcgcg                                 <  2:745809/91‑1 (MQ=255)                                                           tCCGCCGCTGGCTGGCAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGa                           <  1:332183/91‑1 (MQ=255)                                                                     gctggcAGGGCAGAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCAccc                 <  1:622571/91‑1 (MQ=255)                                                                               cagAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGtt       <  1:612540/91‑1 (MQ=255)                                                                               cagAATGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGtt       <  1:6083/91‑1 (MQ=255)                                                                                    tGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGgc  <  2:623984/91‑1 (MQ=255)                                                                                    tGCCCCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGgc  <  2:97496/91‑1 (MQ=255)                                                                                       |                                                                                        GGGACGCTGCAGATTGTGCCAATGCTGGCGATGAGTGATGCCCGCCGCGCGGCGATTATCCGCCGCTGGCTGGCAGGGCAGAATGCACCGATGCCTTCCCGCGACGCGTTGGTGAGGATCTGGCAGGAAGTGGCGCTGGCGCGGGAAGATGCCTCACCCTGTTTACGTTTGGGC  >  NZ_CP009273/209532‑209705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑