Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,712,188	T→C	D26G (GAT→GGT)	pdxH ←	pyridoxamine 5'‑phosphate oxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,712,188	0	T	C	100.0%	50.5 / NA	16	D26G (GAT→GGT)	pdxH	pyridoxamine 5'‑phosphate oxidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/13);  total (3/13)

CACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGCGGCGTAACCCGCCTTTGGTGTATTCACGGCGCAGATGCGCGATTTGCTGCAATTCGTCGTTATCAGACATGGTTTTCTTT  >  NZ_CP009273/1712103‑1712274                                                                                      |                                                                                       caccaTCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGACCgcgg                                                                                   <  1:766121/91‑1 (MQ=255)  accaTCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGACCgcggc                                                                                  <  2:686403/91‑1 (MQ=255)          ggTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGACCGCGGCGGCGTAAc                                                                          <  2:51427/91‑1 (MQ=255)            tAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGACCGCGGCGGCGTAAccc                                                                        <  1:582809/91‑1 (MQ=255)             aGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGCAAAGCGCGGGGGCGTAACCCg                                                                       >  2:139440/1‑91 (MQ=255)                gTCCGCCAGTTTGGCTTCACAAGCCTGAGGGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGGAGGCCGCGGCGGCGGCAccccccc                                                                    >  2:696225/1‑90 (MQ=255)                gTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGGAGACCGCGGCGGCGGCACCCGccc                                                                    >  2:515669/1‑90 (MQ=255)                gTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGAGCGCGGCGGGGTAACGCGCCt                                                                    >  1:85808/1‑91 (MQ=255)                gTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGACCGCGGCGGCGGAACCCGCCt                                                                    >  1:845381/1‑91 (MQ=255)                  ccGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGACCGCGGCGGCGTAACCCGCCctt                                                                  >  1:252116/1‑91 (MQ=255)                   cGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGACCGCGGCGGCGTAACCCGCCTTTg                                                                 <  2:286232/91‑1 (MQ=255)                   cGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGACCGCGGCGGCGGAACCCGCCTTTg                                                                 >  1:255838/1‑91 (MQ=255)                     ccAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGGAGACCGCGGGGGCGGAAACCGCCCTTggg                                                               >  2:509719/1‑90 (MQ=255)                       aGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGACCGCGGGGGCGGAACCCGCCTTTGgtgt                                                             >  1:324148/1‑91 (MQ=255)                                          gagagaGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGACCGCGGCGGCGTAACCCGCCCTTGGTGTATTCACGGCGCAGATgcgc                                          >  1:368618/1‑91 (MQ=255)                                              gagCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGACCGCGGCGGCGTAACCCGCCTTTGGTGTATTCACGGCGCAGATGCGCGAtt                                      <  2:75555/91‑1 (MQ=255)                                                        tCAAAAAGGGTTAATGGATCGGCGGGAAGACCGCGGCGGCGTAACCCGCCTTTGGTGTATTCACGGCGCAGATGCGCGATTTGCTGCAAtt                            <  2:793584/91‑1 (MQ=255)                                                           aaaaGGGTTAATGGATCGGCGGGAAGACCGCGGCGGCGTAACCCGCCTTTGGTGTATTCACGGCGCAGATGCGCGATTTGCTGCAATtcgt                         <  2:691414/91‑1 (MQ=255)                                                             aaGGGTTAATGGATCGGCGGGAAGACCGCGGCGGCGTAACCCGCCTTTGGTGTATTCACGGCGCAGATGCGCGATTTGCTGCAATtcgtcg                       <  1:349265/91‑1 (MQ=255)                                                             aaGGGTTAATGGATCGGCGGGAAGACCGCGGCGGCGTAACCCGCCTTTGGTGTATTCACGGCGCAGATGCGCGATTTGCTGCAATtcgtcg                       <  1:116595/91‑1 (MQ=255)                                                                       ggATCGGCGGGAAGACCGCGGCGGCGTAACCCGCCTTTGGTGTATTCACGGCGCAGATGCGCGATTTGCTGCAATTCGTCGTTATCAGACa             <  2:432837/91‑1 (MQ=255)                                                                             gcggGAAGACCGCGGCGGCGTAACCCGCCTTTGGTGTATTCACGGCGCAGATGCGCGATTTGCTGCAATTCGTCGTTATCAGACATGGttt       <  1:11871/91‑1 (MQ=255)                                                                                  aaGACCGCGGCGGCGTAACCCGCCTTTGGTGGATTCACGGCGCAGATGCGCGATTTGCTGCAATTCGTCGTTATCAGACATGGTTTTCttt  <  1:865834/91‑1 (MQ=255)                                                                                      |                                                                                       CACCATCGCGGTAGGGTCCGCCAGTTTGGCTTCACAAGCCTGAGAGAGCCAACGTTCAAAAAGGGTTAATGGATCGGCGGGAAGATCGCGGCGGCGTAACCCGCCTTTGGTGTATTCACGGCGCAGATGCGCGATTTGCTGCAATTCGTCGTTATCAGACATGGTTTTCTTT  >  NZ_CP009273/1712103‑1712274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑