Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,734,997	T→G	L277R (CTG→CGG)	punC →	purine nucleoside transporter PunC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,734,997	0	T	G	100.0%	85.9 / NA	23	L277R (CTG→CGG)	punC	purine nucleoside transporter PunC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/16);  total (7/16)

TCGCGTTTCTGATTGGTGGTTATGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCAT  >  NZ_CP009273/1734913‑1735074                                                                                     |                                                                              tCGCGTTTCTGATTGGTGGTTATGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGTAGTTATTACCGTGGTTGCTGGTGCGGTTTGc                                                                         <  1:831729/91‑1 (MQ=255)       ttCTGATTGGTGGTTATGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCGGTTTGCTGTCAg                                                                   <  2:811287/91‑1 (MQ=255)               ggtggtTATGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTg                                                           <  2:615169/91‑1 (MQ=255)               ggtggtTATGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTg                                                           <  1:531072/91‑1 (MQ=255)                  ggtTATGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGa                                                        >  1:107132/1‑91 (MQ=255)                           tGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTg                                               <  1:258827/91‑1 (MQ=255)                               gcgccgcgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCggg                                           <  2:639068/91‑1 (MQ=255)                                cgccgcgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGc                                          <  1:371575/91‑1 (MQ=255)                                 gccgcgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCt                                         <  1:273169/91‑1 (MQ=255)                                 gccgcgcTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCt                                         <  2:296118/91‑1 (MQ=255)                                     cgcTGCAGAAATGGCAAGGCAAGCAG‑TATTACCGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCAtt                                    <  1:723384/91‑1 (MQ=255)                                                   caaggcaagCAGTTATTACCGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGc                       >  1:660442/1‑91 (MQ=255)                                                            cAGTTATTACCGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGaaa              <  1:410378/91‑1 (MQ=255)                                                                tattaCCGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCt          <  1:180546/91‑1 (MQ=255)                                                                tattaCCGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCt          <  2:313840/91‑1 (MQ=255)                                                                tattaCCGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCt          <  1:420888/91‑1 (MQ=255)                                                                tattaCCGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCt          <  1:341973/91‑1 (MQ=255)                                                                     ccGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATcc     <  1:55104/91‑1 (MQ=255)                                                                      cGTGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATccc    >  1:381650/1‑91 (MQ=255)                                                                        tGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCAt  >  2:289208/1‑91 (MQ=255)                                                                        tGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCAt  >  2:437638/1‑91 (MQ=255)                                                                        tGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCAt  >  2:757377/1‑91 (MQ=255)                                                                        tGGTTGCTGGTGCGGTTTGCTGTCAGCGTCATTGCGACCAGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCAt  >  1:546285/1‑91 (MQ=255)                                                                                     |                                                                              TCGCGTTTCTGATTGGTGGTTATGGCTGTCGCGCCGCGCTGCAGAAATGGCAAGGCAAGCAGTTATTACCGTGGTTGCTGGTGCTGTTTGCTGTCAGCGTCATTGCGACCTGGGCTGCGGGCTTCATTAGCCATGTGTCGCTGGTCGAAATCCTGATCCCAT  >  NZ_CP009273/1734913‑1735074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑