Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,737,390	T→C	E37G (GAA→GGA)	ribE ←	riboflavin synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,737,390	0	T	C	100.0%	112.6 / NA	30	E37G (GAA→GGA)	ribE	riboflavin synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/17);  total (13/17)

TTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTG  >  NZ_CP009273/1737302‑1737476                                                                                         |                                                                                       ttctttcATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTccc                                                                                      >  2:867805/1‑91 (MQ=255) ttctttcATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTccc                                                                                      >  2:838253/1‑91 (MQ=255)  tctttcATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCa                                                                                     >  2:176832/1‑91 (MQ=255)    tttcATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAgg                                                                                   >  1:657792/1‑91 (MQ=255)     ttcATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGc                                                                                  <  2:80283/91‑1 (MQ=255)            ggTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAg                                                                           >  2:729798/1‑91 (MQ=255)            ggTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAg                                                                           >  2:322663/1‑91 (MQ=255)                aaaaCTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCAtg                                                                       <  1:840123/91‑1 (MQ=255)                  aaCTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCAtgtg                                                                     <  1:466861/91‑1 (MQ=255)                    cTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGt                                                                   <  1:560881/91‑1 (MQ=255)                    cTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGt                                                                   <  2:389347/91‑1 (MQ=255)                         aTGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGt                                                              <  2:314187/91‑1 (MQ=255)                         aTGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGt                                                              <  2:444816/91‑1 (MQ=255)                          ttgTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTa                                                             <  2:578780/89‑1 (MQ=255)                          tGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTa                                                             <  2:720332/91‑1 (MQ=255)                          tGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTa                                                             <  1:649190/91‑1 (MQ=255)                                ccGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTcca                                                       >  1:315164/1‑91 (MQ=255)                                                cGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGaaa                                       >  1:402454/1‑91 (MQ=255)                                                      ggCAGCAACCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTgg                                 >  2:540376/1‑91 (MQ=255)                                                            aaCCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTcc                                                                             >  1:669772/1‑41 (MQ=255)                                                            aaCCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTcc                                                                             <  2:669772/41‑1 (MQ=255)                                                            aaCCGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTtctc                           >  2:238539/1‑91 (MQ=255)                                                               cGTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTc                        <  2:622055/91‑1 (MQ=255)                                                                gTTATGCGCCACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCa                       >  1:167453/1‑91 (MQ=255)                                                                      cgcCACGGAAGCACCGGTTCCCAGGCCGTCCATCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGac                 <  1:650528/91‑1 (MQ=255)                                                                        ccACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGacac               >  2:695986/1‑91 (MQ=255)                                                                         cACGGAAGCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACAcc              <  1:35403/91‑1 (MQ=255)                                                                                gCACCGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTg       >  1:527632/1‑91 (MQ=255)                                                                                   ccGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCgg    <  2:503326/91‑1 (MQ=255)                                                                                   ccGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCgg    <  2:508963/91‑1 (MQ=255)                                                                                   ccGGTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCgg    <  1:695986/91‑1 (MQ=255)                                                                                     ggTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTg  >  2:23396/1‑91 (MQ=255)                                                                                     ggTTCCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTg  >  2:764885/1‑91 (MQ=255)                                                                                         |                                                                                       TTCTTTCATCAGGTCAAAACTGACATGGTTGCCGTTAATTTCCGTCACGGTCAGGCAGCAACCGTTATGCGCCACGGAAGCACCGGTTTCCAGGCCGTCCAGCATGTGGTCGGGTAACTCCACCACATGCGTACGAAAATTTGGTTTCTCGTCAATCGACACCAGTTTTGCGGTG  >  NZ_CP009273/1737302‑1737476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑