Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,765,154	A→G	N95D (AAT→GAT)	ydiL →	YdiL family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,765,154	0	A	G	100.0%	109.5 / NA	30	N95D (AAT→GAT)	ydiL	YdiL family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/17);  total (13/17)

CATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAA  >  NZ_CP009273/1765066‑1765241                                                                                         |                                                                                        catAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATAGCGAt                                                                                       >  2:361637/1‑91 (MQ=255)     gagaAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTa                                                                                   >  2:636881/1‑91 (MQ=255)      agaAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTAt                                                                                  <  1:217974/91‑1 (MQ=255)         aaataaatAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGa                                                                               >  1:188130/1‑91 (MQ=255)           ataaataaCCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACt                                                                             <  1:661489/91‑1 (MQ=255)                     gCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTa                                                                   >  2:92977/1‑91 (MQ=255)                     gCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTa                                                                   >  1:674795/1‑91 (MQ=255)                      cATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTAt                                                                  >  1:388862/1‑91 (MQ=255)                       aTCGGCAACAACACCATGCGTTTTTTCCCCGACTTACCCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGCAAGATCTATc                                                                 >  2:813978/1‑91 (MQ=255)                             aacaacACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCgg                                                           >  1:302153/1‑91 (MQ=255)                               caacaCCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGCTa                                                         <  2:157061/91‑1 (MQ=255)                                 acacCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAgc                                                       <  1:402855/91‑1 (MQ=255)                                 acacCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAgc                                                       <  1:203712/91‑1 (MQ=255)                                    ccATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAgctgc                                                    <  2:302153/91‑1 (MQ=255)                                     cATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAgctgct                                                   >  1:562728/1‑91 (MQ=255)                                     cATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAgc                                                       <  2:738892/87‑1 (MQ=255)                                     cATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAgc                                                       >  1:738892/1‑87 (MQ=255)                                        gCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGaa                                                <  1:780509/91‑1 (MQ=255)                                               ttCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATg                                         <  1:569033/91‑1 (MQ=255)                                                   ccGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGcaca                                     >  1:804175/1‑91 (MQ=255)                                                    cGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCacac                                    <  1:40148/91‑1 (MQ=255)                                                    cGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCacac                                    <  2:562728/91‑1 (MQ=255)                                                    cGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCacac                                    <  1:374789/91‑1 (MQ=255)                                                    cGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCacac                                    <  1:584435/91‑1 (MQ=255)                                                    cGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTACATGCacac                                    <  2:860328/91‑1 (MQ=255)                                                              gCATTTCAACGAGTCTACCCTGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGc                          <  1:155289/91‑1 (MQ=255)                                                                                  tGATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCcac      >  1:763854/1‑91 (MQ=255)                                                                                   gATGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCcaca     >  1:411850/1‑91 (MQ=255)                                                                                    aTGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAg    >  1:851708/1‑91 (MQ=255)                                                                                    aTGGCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAg    <  2:763854/91‑1 (MQ=255)                                                                                      ggCGATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGaa  <  1:760600/91‑1 (MQ=255)                                                                                         |                                                                                        CATAGAGAAAATAAATAACCGCATCGGCAACAACACCATGCGTTTTTTCCCCGACTTAACCGCATTTCAACGAGTCTACCCTGATGGCAATTTTATTGACTGGAAGATCTATCAATCGGTAGCTGCTGAATTATATGCACACGATCTGGAGCGGCTTTGTTAATTTTTCCACAGAA  >  NZ_CP009273/1765066‑1765241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑