Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,769,436	A→G	D165G (GAC→GGC)	aroD →	type I 3‑dehydroquinate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,769,436	0	A	G	100.0%	126.3 / NA	35	D165G (GAC→GGC)	aroD	type I 3‑dehydroquinate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/24);  total (11/24)

AAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGACGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCA  >  NZ_CP009273/1769352‑1769523                                                                                     |                                                                                        aaGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGa                                                                                   <  2:772697/91‑1 (MQ=255)   gtagtCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGata                                                                                 <  2:451985/91‑1 (MQ=255)   gtagtCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGata                                                                                 <  2:795076/91‑1 (MQ=255)   gtagtCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGata                                                                                 <  1:631984/91‑1 (MQ=255)   gtagtCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGata                                                                                 <  1:423510/91‑1 (MQ=255)   gtagtCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGata                                                                                 <  1:540694/91‑1 (MQ=255)   gtagtCATGACCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGata                                                                                 <  2:280984/91‑1 (MQ=255)     agtCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATAtt                                                                               >  2:484570/1‑91 (MQ=255)     agtCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATAtt                                                                               >  2:440814/1‑91 (MQ=255)     agtCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATAtt                                                                               >  2:321726/1‑91 (MQ=255)       tCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTcc                                                                             >  1:404916/1‑91 (MQ=255)          tGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTaa                                                                          >  2:426115/1‑91 (MQ=255)                   aTGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCt                                                                 <  1:152087/91‑1 (MQ=255)                    tGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGGTTGCGCTg                                                                >  2:152664/1‑91 (MQ=255)                    tGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTg                                                                >  2:650042/1‑91 (MQ=255)                        ttCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGc                                                            <  1:440814/91‑1 (MQ=255)                             tAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCaa                                                       <  2:795988/91‑1 (MQ=255)                              aaaaCGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCaaa                                                      <  2:591422/91‑1 (MQ=255)                                 aCGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTa                                                   >  2:687300/1‑91 (MQ=255)                                    ccGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCa                                                >  1:821366/1‑91 (MQ=255)                                        aaGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGa                                            <  2:406088/91‑1 (MQ=255)                                                          cccGTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGttgcttgc                          >  1:856417/1‑91 (MQ=255)                                                             gTCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCcgc                       <  2:328153/91‑1 (MQ=255)                                                              tCTGCGCAAAATGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCcgcg                      <  2:204938/91‑1 (MQ=255)                                                                      aaaTGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGga              <  2:211503/91‑1 (MQ=255)                                                                       aaTGCAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGgag             <  2:733600/91‑1 (MQ=255)                                                                           cAATCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGAttc         >  1:310354/1‑89 (MQ=255)                                                                            aaTCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATTCa        <  2:310354/91‑1 (MQ=255)                                                                              tCCTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAgg      <  1:708842/91‑1 (MQ=255)                                                                                cTTCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAg    <  1:669928/91‑1 (MQ=255)                                                                                 ttCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGc   <  2:54562/91‑1 (MQ=255)                                                                                  tCGGCGCCGATATTCCTAAGGTTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCa  <  1:152664/91‑1 (MQ=255)                                                                                  tCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCaa                                                       <  1:533658/38‑1 (MQ=255)                                                                                  tCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCa  <  1:650042/91‑1 (MQ=255)                                                                                  tCGGCGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCa  <  2:534541/91‑1 (MQ=255)                                                                                     |                                                                                        AAGTAGTCATGTCCAACCATGACTTCCATAAAACGCCGGAAGCCGAAGAAATCATTGCCCGTCTGCGCAAAATGCAATCCTTCGACGCCGATATTCCTAAGATTGCGCTGATGCCGCAAAGTACCAGCGATGTGCTGACGTTGCTTGCCGCGACCCTGGAGATGCAGGAGCA  >  NZ_CP009273/1769352‑1769523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑