Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,776,037	T→G	V129G (GTT→GGT)	ydiS →	FAD‑dependent oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,776,037	0	T	G	100.0%	96.9 / NA	28	V129G (GTT→GGT)	ydiS	FAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/9);  total (19/9)

CTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTC  >  NZ_CP009273/1775956‑1776123                                                                                  |                                                                                       cTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGa                                                                               <  1:830127/91‑1 (MQ=255)        cGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTg                                                                        >  1:425026/1‑91 (MQ=255)         gaaTTGGGTAATCGCCTGGCCCCGGGGTTAATGGAACAAGCCGAGCAGGCGGGCGCGCGGTTTATCCCAGGCGGTCGCGTCGAGGCGCggg                                                                       >  2:208186/3‑91 (MQ=255)         gTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTgg                                                                       >  2:634046/1‑91 (MQ=255)         gTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTgg                                                                       >  1:527654/1‑91 (MQ=255)         gTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGACCAGGCGGGCGCGCCGTTTATACCAGGCGGTCGGGTCCACGCGCTgg                                                                       >  2:735014/1‑91 (MQ=255)          tATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGt                                                                      >  1:408555/1‑91 (MQ=255)           aTTGCGTACTCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCCGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGtt                                                                     >  2:520682/1‑91 (MQ=255)           aTTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGtt                                                                     >  2:435236/1‑91 (MQ=255)                 tAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGaa                                                               >  2:290996/1‑91 (MQ=255)                  aaTCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAg                                                              >  1:833803/1‑91 (MQ=255)                   aTCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGt                                                                  <  2:813182/86‑1 (MQ=255)                   aTCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGt                                                                  >  1:813182/1‑86 (MQ=255)                   aTCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAgg                                                             >  1:444419/1‑91 (MQ=255)                        ccggACCCGTGGTTGAGGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACa                                                        <  1:426693/89‑1 (MQ=255)                        cTGGACCCGTGGTTGATGGAACAAGCCGAGCGGGCGGGCGCGCAGTTTATCCCAGGCGGGCGCGGCGACGGCCTTGTTTGTGGAGGgaaaa                                                        >  2:806516/1‑89 (MQ=255)                        cTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACa                                                        >  2:261816/1‑91 (MQ=255)                                gTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACt                                                <  2:527654/91‑1 (MQ=255)                                       aTGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGc                                         <  2:214970/91‑1 (MQ=255)                                       aTGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGc                                         <  2:695343/91‑1 (MQ=255)                                          gAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAgg                                      >  2:118966/1‑91 (MQ=255)                                           aaCAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGc                                     <  1:634046/91‑1 (MQ=255)                                             cAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTg                                   <  1:118966/91‑1 (MQ=255)                                              aaGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTgg                                  >  1:223235/1‑91 (MQ=255)                                                   gAGCAGGCGGGCGCGCAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGgatg                             >  1:176267/1‑91 (MQ=255)                                                             gcgcgcAGTTTATCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGa                   >  1:79373/1‑91 (MQ=255)                                                                         tCCCAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATgtggt       >  2:310337/1‑91 (MQ=255)                                                                          cccAGGCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATgtggtg      <  2:387086/91‑1 (MQ=255)                                                                              ggCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTc  >  2:520684/1‑91 (MQ=255)                                                                              ggCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTc  >  1:601517/1‑91 (MQ=255)                                                                              ggCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTc  >  2:751538/1‑91 (MQ=255)                                                                              ggCGGTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGGGATTc  >  1:93355/1‑91 (MQ=255)                                                                                  |                                                                                       CTTATACCGTATTGCGTAATCGTCTGGACCCGTGGTTGATGGAACAAGCCGAGCAGGCGGGCGCGCAGTTTATCCCAGGCGTTCGCGTCGACGCGCTGGTTCGTGAAGGAAACAAGGTCACTGGCGTGCAGGCTGGGGATGATATTCTCGAAGCGAATGTGGTGATTC  >  NZ_CP009273/1775956‑1776123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑