Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	232,843	T→C	I97T (ATC→ACC)	dnaQ →	DNA polymerase III subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	232,843	0	T	C	100.0%	132.2 / NA	35	I97T (ATC→ACC)	dnaQ	DNA polymerase III subunit epsilon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/14);  total (21/14)

TTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTT  >  NZ_CP009273/232756‑232924                                                                                        |                                                                                  ttGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCa                                                                                >  2:449298/1‑91 (MQ=255) ttGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCa                                                                                >  1:369079/1‑91 (MQ=255) ttGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCa                                                                                >  1:389164/1‑91 (MQ=255)      gATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACg                                                                           >  1:386952/1‑91 (MQ=255)       aTGAATTTTTGCTCGATCCGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGc                                                                          >  2:485149/1‑91 (MQ=255)       aTGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGc                                                                          <  2:706287/91‑1 (MQ=255)        tGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCa                                                                         >  2:477965/1‑91 (MQ=255)             ttttGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGtt                                                                    <  1:665399/91‑1 (MQ=255)             ttttGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGtt                                                                    >  2:584674/1‑91 (MQ=255)                    cGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATc                                                             <  2:656954/91‑1 (MQ=255)                         aGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCtt                                                        <  1:816314/91‑1 (MQ=255)                            ccACGTTTGCCGAAGTAGCCGATGAGGTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTAt                                                     <  1:28775/91‑1 (MQ=255)                                             gCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGc                                                                          >  1:526076/1‑53 (MQ=255)                                             gCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGc                                                                          <  2:526076/53‑1 (MQ=255)                                             gCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGt                                    >  1:175278/1‑91 (MQ=255)                                                        cATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcgc                         <  2:533168/91‑1 (MQ=255)                                                        cATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcgc                         <  1:764717/91‑1 (MQ=255)                                                        cATGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcgc                         <  1:17874/91‑1 (MQ=255)                                                          tGGACTATATTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGa                       >  1:453584/1‑91 (MQ=255)                                                               tataTTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTAt                                                     >  2:198334/1‑56 (MQ=255)                                                               tataTTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTAt                                                     <  1:198334/56‑1 (MQ=255)                                                               tataTTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTc                  >  2:620756/1‑91 (MQ=255)                                                               tataTTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTc                  >  2:311490/1‑91 (MQ=255)                                                                atatTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTcc                 >  2:312458/1‑91 (MQ=255)                                                                atatTCGCGGCGCGGAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTcc                 >  1:723365/1‑91 (MQ=255)                                                                      gcggcgcggAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGAc           >  2:258448/1‑91 (MQ=255)                                                                      gcggcgcggAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGAc           >  1:650575/1‑91 (MQ=255)                                                                      gcggcgcggAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCAAAGAc           >  1:321795/1‑91 (MQ=255)                                                                        ggcgcggAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCa         >  1:828713/1‑91 (MQ=255)                                                                          cgcggAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa               >  1:797083/1‑83 (MQ=255)                                                                          cgcggAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGa               <  2:797083/83‑1 (MQ=255)                                                                          cgcggAGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAAt       <  2:293358/91‑1 (MQ=255)                                                                               aGTTGGTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACttt  <  2:504661/91‑1 (MQ=255)                                                                                   ggTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcgc                         <  2:590709/64‑1 (MQ=255)                                                                                   ggTGACCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAgcgc                         >  1:590709/1‑64 (MQ=255)                                                                                        |                                                                                  TTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTT  >  NZ_CP009273/232756‑232924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑