Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,809,395	T→G	G424G (GGT→GGG)	katE →	catalase HPII

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,809,395	0	T	G	100.0%	110.3 / NA	30	G424G (GGT→GGG)	katE	catalase HPII
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/13);  total (17/13)

TATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGA  >  NZ_CP009273/1809308‑1809480                                                                                        |                                                                                      tatCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTgggggg                                                                                    >  2:569355/1‑91 (MQ=255)   tCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGcc                                                                                  >  1:655069/1‑91 (MQ=255)    cGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCg                                                                                 <  1:186818/91‑1 (MQ=255)       gCCGGGACT‑GACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAAtt                                                                             <  2:305542/91‑1 (MQ=255)                  actTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGAt                                                                   <  1:30110/91‑1 (MQ=255)                  actTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGAt                                                                   <  1:513194/91‑1 (MQ=255)                  actTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGAt                                                                   <  2:538726/91‑1 (MQ=255)                            cGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAAc                                                         <  1:118379/91‑1 (MQ=255)                                ccGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTc                                                     <  1:414057/91‑1 (MQ=255)                                      ttGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCt                                               >  1:394676/1‑91 (MQ=255)                                        gCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGc                                             >  1:842222/1‑91 (MQ=255)                                                         ccTATACCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCa                            >  2:99871/1‑91 (MQ=255)                                                            ataCCGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCg                         >  2:179253/1‑91 (MQ=255)                                                                cGATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGAc                     <  1:707312/91‑1 (MQ=255)                                                                 gATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTc                                                     <  2:643865/58‑1 (MQ=255)                                                                 gATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTc                                                     >  1:643865/1‑58 (MQ=255)                                                                 gATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGAcc                                                >  1:773078/1‑63 (MQ=255)                                                                 gATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGAcc                                                <  2:773078/63‑1 (MQ=255)                                                                 gATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACg                    >  1:180701/1‑91 (MQ=255)                                                                 gATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACg                    >  2:764505/1‑91 (MQ=255)                                                                 gATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACg                    >  2:612164/1‑91 (MQ=255)                                                                 gATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACg                    >  1:563823/1‑91 (MQ=255)                                                                 gATACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACg                    >  2:185723/1‑91 (MQ=255)                                                                  aTACACAAATCAGTCGTCTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACgg                   <  2:842222/91‑1 (MQ=255)                                                                         aaTCAGTCGTCTTGGGGGGCCGCATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGgcatgcat            >  1:742084/1‑91 (MQ=255)                                                                              gtcgtcTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCAt       <  2:21997/91‑1 (MQ=255)                                                                              gtcgtcTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCAt       <  1:77439/91‑1 (MQ=255)                                                                                 gtcTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATggg    >  2:475725/1‑91 (MQ=255)                                                                                   cTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGa  >  2:317735/1‑91 (MQ=255)                                                                                   cTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGa  >  2:261080/1‑91 (MQ=255)                                                                                   cTTGGGGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGa  >  1:716426/1‑91 (MQ=255)                                                                                        |                                                                                      TATCGTGCCGGGACTGGACTTCACCAACGATCCGCTGTTGCAGGGACGTTTGTTCTCCTATACCGATACACAAATCAGTCGTCTTGGTGGGCCGAATTTCCATGAGATTCCGATTAACCGTCCGACCTGCCCTTACCATAATTTCCAGCGTGACGGCATGCATCGCATGGGGA  >  NZ_CP009273/1809308‑1809480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑