Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,810,989	A→G	F135S (TTC→TCC)	chbG ←	chitin disaccharide deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,810,989	0	A	G	100.0%	109.5 / NA	29	F135S (TTC→TCC)	chbG	chitin disaccharide deacetylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/21);  total (8/21)

GGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTG  >  NZ_CP009273/1810906‑1811074                                                                                    |                                                                                      ggTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTg                                                                                >  2:9657/1‑91 (MQ=255)    tGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCgg                                                                             <  2:808366/91‑1 (MQ=255)    tGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCgg                                                                             <  1:509402/91‑1 (MQ=255)      aCCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGa                                                                           <  1:533832/91‑1 (MQ=255)      aCCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGa                                                                           <  1:648197/91‑1 (MQ=255)      aCCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGa                                                                           <  1:24786/91‑1 (MQ=255)       ccGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGaa                                                                          <  1:4219/91‑1 (MQ=255)              aTCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGc                                                                   <  1:546539/91‑1 (MQ=255)                        aTCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACatgatgat                                                         >  2:617315/1‑91 (MQ=255)                        aTCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACatgatgat                                                         <  2:54267/91‑1 (MQ=255)                         tCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACatgatgatg                                                        <  2:748590/91‑1 (MQ=255)                         tCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACatgatgatg                                                        <  1:163289/91‑1 (MQ=255)                         tCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACatgatgatg                                                        <  2:359103/91‑1 (MQ=255)                           tGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGc                                                      <  1:582405/91‑1 (MQ=255)                              cGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTAt                                                   >  2:801277/1‑91 (MQ=255)                                   cgcccgcAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGa                                              <  1:316586/91‑1 (MQ=255)                                    gcccgcAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGAt                                             >  2:450707/1‑91 (MQ=255)                                              caaTGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGttt                                   <  2:297826/91‑1 (MQ=255)                                              caaTGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGttt                                   <  2:61325/91‑1 (MQ=255)                                                     cTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACatgatgatg                                                        >  2:400808/1‑63 (MQ=255)                                                     cTGCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACatgatgatg                                                        <  1:400808/63‑1 (MQ=255)                                                       gCTCTGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCaaa                          >  1:54726/1‑91 (MQ=255)                                                        ctctGCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAAt                         <  2:586228/91‑1 (MQ=255)                                                            gCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATc                                                  <  2:410164/62‑1 (MQ=255)                                                            gCCGCAAACCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATc                                                  >  1:410164/1‑62 (MQ=255)                                                                 aaaCCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATg                >  1:187468/1‑91 (MQ=255)                                                                   aCCTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGaa              <  2:787289/91‑1 (MQ=255)                                                                     cTGGCAACAATCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAAc            <  1:16362/91‑1 (MQ=255)                                                                               tCGGGGAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATactg  <  2:54726/91‑1 (MQ=255)                                                                                    |                                                                                      GGTTGACCGGCAAATCAAACGCCATCTGACGATCCGCCCGCAACGCAATGCCCTGCTCTGCCGCAAACCTGGCAACAATCGGGAAAATCTGCGGGAACATATGCACATGATGATGGCTATCAAGATGCGTAGGTTTGCGTCCAAATAGCTCAATGAAACGCAAATACTG  >  NZ_CP009273/1810906‑1811074

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑