Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,834,793	A→G	D357G (GAT→GGT)	ynjE →	thiosulfate sulfurtransferase YnjE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,834,793	0	A	G	100.0%	84.2 / NA	23	D357G (GAT→GGT)	ynjE	thiosulfate sulfurtransferase YnjE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/12);  total (11/12)

ATATTAAACCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGATGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGC  >  NZ_CP009273/1834706‑1834878                                                                                        |                                                                                      atatTAAACCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTgg                                                                                    <  2:521648/91‑1 (MQ=255)      aaaCCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCa                                                                               >  2:83554/1‑91 (MQ=255)      aaaCCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCa                                                                               <  2:783812/91‑1 (MQ=255)      aaaCCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCa                                                                               <  2:42339/91‑1 (MQ=255)        aCCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATg                                                                             >  1:596724/1‑91 (MQ=255)           aaaaGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATgcgc                                                                          >  1:33533/1‑91 (MQ=255)           aaaaGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATgcgc                                                                          >  2:657218/1‑91 (MQ=255)           aaaaGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATgcgc                                                                          >  2:599909/1‑91 (MQ=255)                     aTAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCgatg                                                                <  2:451381/91‑1 (MQ=255)                     aTAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCgatg                                                                <  2:678986/91‑1 (MQ=255)                       aGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCgatgat                                                              <  1:189345/91‑1 (MQ=255)                            gcgcACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTAc                                                         <  1:134088/91‑1 (MQ=255)                            gcgcACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTAc                                                         >  2:47485/1‑91 (MQ=255)                            gcgcACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTAc                                                         >  1:396236/1‑91 (MQ=255)                                  gTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTAt                                                   <  1:571433/91‑1 (MQ=255)                                            cGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCg                                         >  2:43521/1‑91 (MQ=255)                                                    gCGACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAatat                                 >  1:624521/1‑91 (MQ=255)                                                      gACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATc                                <  2:172146/90‑1 (MQ=255)                                                      gACTCGACGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATc                                >  1:172146/1‑90 (MQ=255)                                                            aCGCATATGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCag                         >  2:708944/1‑91 (MQ=255)                                                                 tatGGAAGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGagcag                    >  2:493791/1‑91 (MQ=255)                                                                      aaGATTTTCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGt               <  2:568702/91‑1 (MQ=255)                                                                           tttCATAACCCGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCAt          <  2:515988/91‑1 (MQ=255)                                                                                   cccGGGTGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGc  <  2:239558/91‑1 (MQ=255)                                                                                        |                                                                                      ATATTAAACCAAAAGGTGAAATAGCCGGCGCACGTTGGGGACACGCTGGTAGCGACTCGACGCATATGGAAGATTTTCATAACCCGGATGGCACCATGCGCAGCGCCGATGATATTACCGCTATGTGGAAAGCGTGGAATATCAAACCAGAGCAGCAAGTTTCATTCTACTGC  >  NZ_CP009273/1834706‑1834878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑