Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,879,784	T→G	V288G (GTG→GGG)	yeaW →	carnitine monooxygenase subunit YeaW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,879,784	0	T	G	100.0%	150.9 / NA	40	V288G (GTG→GGG)	yeaW	carnitine monooxygenase subunit YeaW
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (19/21);  total (19/21)

CCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGT  >  NZ_CP009273/1879701‑1879867                                                                                    |                                                                                    ccACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCt                                                                              <  1:769559/91‑1 (MQ=255)      gTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAAc                                                                         <  1:440926/91‑1 (MQ=255)          cTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTAcc                                                                     <  1:323556/91‑1 (MQ=255)            ggCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGACATTTATGAATTCCCGGGGGATTCTGAAAGCACCCt                                                                   >  2:436774/1‑91 (MQ=255)              cTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGc                                                                 <  1:703185/91‑1 (MQ=255)                gtgGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCaa                                                               >  1:736381/1‑91 (MQ=255)                      gTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTAc                                                         >  2:815131/1‑91 (MQ=255)                      gTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTAc                                                         <  2:406533/91‑1 (MQ=255)                        gCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGa                                                       <  2:65554/91‑1 (MQ=255)                           cGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGatat                                                    <  2:781013/91‑1 (MQ=255)                           cGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGatat                                                    <  2:516560/91‑1 (MQ=255)                             aTGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATAttt                                                  >  2:808742/1‑91 (MQ=255)                                  gAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTc                                             <  2:28105/91‑1 (MQ=255)                                  gAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTc                                             <  2:85834/91‑1 (MQ=255)                                       tCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGAtt                                                                                <  1:331166/51‑1 (MQ=255)                                       tCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGAtt                                                                                >  2:331166/1‑51 (MQ=255)                                       tCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGAtt                                                                                <  1:268951/51‑1 (MQ=255)                                       tCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGAtt                                                                                >  2:268951/1‑51 (MQ=255)                                       tCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCaa                                        >  2:337441/1‑91 (MQ=255)                                       tCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCaa                                        >  2:532571/1‑91 (MQ=255)                                       tCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCaa                                        >  1:106818/1‑91 (MQ=255)                                       tCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCaa                                        >  1:569474/1‑91 (MQ=255)                                         aCCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATg                                      >  1:607721/1‑91 (MQ=255)                                            ccGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAag                                   <  2:325476/91‑1 (MQ=255)                                              gATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAagag                                 >  1:682534/1‑91 (MQ=255)                                              gATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAagag                                 >  2:40010/1‑91 (MQ=255)                                                       gatgatGACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACcgac                        <  2:790833/91‑1 (MQ=255)                                                        atgatgACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACg                                                        >  1:773121/1‑58 (MQ=255)                                                        atgatgACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACg                                                        <  2:773121/58‑1 (MQ=255)                                                        atgatgACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACcgacg                       >  2:178122/1‑91 (MQ=255)                                                        atgatgACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACcgacg                       >  1:518068/1‑91 (MQ=255)                                                        atgatgACGGTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACcgacg                       >  1:206646/1‑91 (MQ=255)                                                                ggTCATTTATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCaaaaa               >  1:467037/1‑91 (MQ=255)                                                                      ttATGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTg         <  1:120021/91‑1 (MQ=255)                                                                        aTGAATTCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGAt       <  1:243995/91‑1 (MQ=255)                                                                             ttCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGt  <  2:732773/91‑1 (MQ=255)                                                                             ttCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGt  >  2:146720/1‑91 (MQ=255)                                                                             ttCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGt  >  1:7251/1‑91 (MQ=255)                                                                             ttCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGt  <  1:507655/91‑1 (MQ=255)                                                                             ttCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGt  <  2:840840/91‑1 (MQ=255)                                                                             ttCCCGGGGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGt  <  1:121900/91‑1 (MQ=255)                                                                                    |                                                                                    CCACGGTTTCTGGCTGTGGCCGTGCACGATGCTGAACGTCACCCCGATCAAAGGGATGATGACGGTCATTTATGAATTCCCGGTGGATTCTGAAACTACCCTGCAAAACTACGATATTTACTTCACCAATGAAGAGTTAACCGACGAGCAAAAATCGCTGATTGAGT  >  NZ_CP009273/1879701‑1879867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑