Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,900,350	A→G	T136A (ACC→GCC)	mntP →	manganese efflux pump MntP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,900,350	0	A	G	100.0%	72.6 / NA	20	T136A (ACC→GCC)	mntP	manganese efflux pump MntP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/12);  total (8/12)

CTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCT  >  NZ_CP009273/1900263‑1900422                                                                                        |                                                                         cTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCg                                                                       >  2:224469/1‑91 (MQ=255)    cTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCAt                                                                    <  1:742494/91‑1 (MQ=255)     tGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCAtt                                                                   <  2:196877/91‑1 (MQ=255)            caccGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCAtt                                                            <  1:629968/91‑1 (MQ=255)              ccGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTgg                                                          >  2:327762/1‑91 (MQ=255)              ccGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTgg                                                          >  1:418220/1‑91 (MQ=255)                     tGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCAtt                                                                   >  2:516872/1‑75 (MQ=255)                     tGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCAtt                                                                   <  1:516872/75‑1 (MQ=255)                             gCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGAt                                           >  2:252668/1‑91 (MQ=255)                                   aTGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTCTGCAACCTTGATTATGTc                                     <  1:822795/91‑1 (MQ=255)                                    tGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCa                                    <  2:99392/91‑1 (MQ=255)                                    tGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCa                                    <  1:327762/91‑1 (MQ=255)                                    tGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCa                                    <  1:224469/91‑1 (MQ=255)                                                    gtTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCa                                                              <  2:140394/49‑1 (MQ=255)                                                    gtTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCa                                                              >  1:140394/1‑49 (MQ=255)                                                    gtTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGAtgg                    >  1:682559/1‑91 (MQ=255)                                                        gTCTTGCTTTCCTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGAtggttgg                <  2:279558/91‑1 (MQ=255)                                                                  ccTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATc      <  1:252668/91‑1 (MQ=255)                                                                  ccTGCAGGTCAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATc      <  2:418220/91‑1 (MQ=255)                                                                      cAGGACAACATTATCGCGGCCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCt  >  1:700535/1‑91 (MQ=255)                                                                                        |                                                                         CTACTGGTAACCACCGCGATTGCCACCAGCCTGGATGCCATGGCTGTGGGTGTTGGTCTTGCTTTCCTGCAGGTCAACATTATCGCGACCGCATTGGCCATTGGTTGTGCAACCTTGATTATGTCAACATTAGGGATGATGGTTGGTCGCTTTATCGGCT  >  NZ_CP009273/1900263‑1900422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑