Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,932,990	A→G	E362G (GAA→GGA)	pyk →	pyruvate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,932,990	0	A	G	100.0%	116.6 / NA	31	E362G (GAA→GGA)	pyk	pyruvate kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (12/19);  total (12/19)

GCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGG  >  NZ_CP009273/1932902‑1933071                                                                                         |                                                                                  gCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGggag                                                                                 <  2:236658/91‑1 (MQ=255)     tGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGc                                                                             <  1:457944/91‑1 (MQ=255)          cgcgTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTg                                                                        <  2:51778/91‑1 (MQ=255)            cgTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGcc                                                                      <  2:139673/91‑1 (MQ=255)                 gCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTc                                                                 >  1:211821/1‑91 (MQ=255)                 gCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTc                                                                 <  1:295564/91‑1 (MQ=255)                   cTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAg                                                               <  2:94571/91‑1 (MQ=255)                      ggTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCaa                                                            >  2:390050/1‑91 (MQ=255)                          cGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTa                                                        >  2:15709/1‑91 (MQ=255)                           ggAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTAc                                                       <  1:99155/91‑1 (MQ=255)                             aaaaaaTCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGc                                                     <  1:390050/91‑1 (MQ=255)                                  aTCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTa                                                <  2:468734/91‑1 (MQ=255)                                             cAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGaaa                                     <  2:792644/91‑1 (MQ=255)                                                       aaaCACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACgg                           <  1:521691/91‑1 (MQ=255)                                                        aaCACCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGc                          <  1:687879/91‑1 (MQ=255)                                                         acacCGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCg                         <  2:685631/91‑1 (MQ=255)                                                            ccGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGatc                      >  1:429465/1‑91 (MQ=255)                                                             cGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGatca                     <  2:364349/91‑1 (MQ=255)                                                             cGTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGatca                     <  1:355462/91‑1 (MQ=255)                                                              gTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACgg                           >  1:730419/1‑84 (MQ=255)                                                              gTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACgg                           <  2:730419/84‑1 (MQ=255)                                                              gTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGatcat                    >  2:162926/1‑91 (MQ=255)                                                              gTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGatcat                    >  1:706752/1‑91 (MQ=255)                                                              gTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGatcat                    >  2:417064/1‑91 (MQ=255)                                                              gTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGatcat                    >  1:600691/1‑91 (MQ=255)                                                              gTCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAAAGTAAGCAGGTAAACACCACAAAGGCGGTAAGGGGatcat                    >  2:637044/1‑91 (MQ=255)                                                               tCTGGACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGatcatc                   <  2:645961/91‑1 (MQ=255)                                                                   gACGTTCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCa               <  1:519062/91‑1 (MQ=255)                                                                       ttCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGAc           <  1:15709/91‑1 (MQ=255)                                                                       ttCAGTTCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGAc           <  1:560258/91‑1 (MQ=255)                                                                            ttCGACAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGTAATGTGTTACGGCGGTCCCCACCATGACCGAAt      <  2:811584/91‑1 (MQ=255)                                                                                aCAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCggg  >  1:464394/1‑91 (MQ=255)                                                                                aCAATGTGGGAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCggg  >  1:135064/1‑91 (MQ=255)                                                                                         |                                                                                  GCCATGGCGCGCGTTTGCCTGGGTGCGGAAAAAATCCCGAGCATCAACGTTTCTAAACACCGTCTGGACGTTCAGTTCGACAATGTGGAAGAAGCTATTGCCATGTCAGCAATGTACGCAGCTAACCACCTGAAAGGCGTTACGGCGATCATCACCATGACCGAATCGGG  >  NZ_CP009273/1932902‑1933071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑