Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,941,091	A→G	E195G (GAA→GGA)	yebB →	YebB family permuted papain‑like enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,941,091	0	A	G	100.0%	113.5 / NA	30	E195G (GAA→GGA)	yebB	YebB family permuted papain‑like enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/15);  total (15/15)

GAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGA  >  NZ_CP009273/1941003‑1941172                                                                                         |                                                                                  gAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGAc                                                                                 <  2:288028/91‑1 (MQ=255)       aaaaCCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCg                                                                           >  2:561427/1‑91 (MQ=255)           ccGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGc                                                                       <  2:692419/91‑1 (MQ=255)                aCGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGtat                                                                  <  2:317390/91‑1 (MQ=255)                     agccagTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACtt                                                             >  2:408838/1‑91 (MQ=255)                              gtgGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTc                                                    <  2:363652/91‑1 (MQ=255)                               tgGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCg                                                   <  2:13801/91‑1 (MQ=255)                                  catcatCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGccc                                                <  1:219102/91‑1 (MQ=255)                                    tcatcCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCctc                                              <  2:619119/91‑1 (MQ=255)                                    tcatcCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCctc                                              >  2:370497/1‑91 (MQ=255)                                               tGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTc                                   >  2:837853/1‑91 (MQ=255)                                               tGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTc                                   <  1:379333/91‑1 (MQ=255)                                               tGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTc                                   <  1:387626/91‑1 (MQ=255)                                                ggTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCa                                  >  1:480620/1‑91 (MQ=255)                                                   gtTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGcc                               <  2:669175/91‑1 (MQ=255)                                                     tGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCcgc                             >  1:124115/1‑91 (MQ=255)                                                           aTGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCg                       >  2:432242/1‑91 (MQ=255)                                                           aTGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACACAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCg                       >  2:635145/1‑91 (MQ=255)                                                            tGCGGAGGGAGTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACACAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGc                      <  2:580335/91‑1 (MQ=255)                                                                gagggagTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCAt                  >  2:346640/1‑91 (MQ=255)                                                                 agggagTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATc                 <  1:467253/91‑1 (MQ=255)                                                                 agggagTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATc                 <  2:518618/91‑1 (MQ=255)                                                                  gggagTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGa                           >  2:372524/1‑80 (MQ=255)                                                                  gggagTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGa                           <  1:372524/80‑1 (MQ=255)                                                                   ggagTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTg               <  2:742131/91‑1 (MQ=255)                                                                    gagTGGAAACGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGc              >  2:115468/1‑91 (MQ=255)                                                                          aaaCGCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCa        >  1:562389/1‑91 (MQ=255)                                                                              gCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCt    >  1:39541/1‑91 (MQ=255)                                                                              gCCTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCt    >  1:653615/1‑91 (MQ=255)                                                                                cTCAGCCGGGACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGa  >  1:71350/1‑91 (MQ=255)                                                                                         |                                                                                  GAGCGTAAAACCGTCACGCCAGCCAGTTTGTGGCATCATCCGGGTTTGGTGTTGATTCATGCGGAGGGAGTGGAAACGCCTCAGCCGGAACTGACCGAGGCGGTATAACTTAACGCAGTCGCCCTCTCGCCAGGTTCAGCCGCGATTCGCTCATCTGCATCGCATTCTGA  >  NZ_CP009273/1941003‑1941172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑