Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,955,016	A→G	D233G (GAC→GGC)	argS →	arginine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,955,016	0	A	G	100.0%	109.7 / NA	30	D233G (GAC→GGC)	argS	arginine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/12);  total (18/12)

GAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGACATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACA  >  NZ_CP009273/1954928‑1955102                                                                                         |                                                                                       gaagaGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCa                                                                                      >  2:128245/1‑91 (MQ=255) gaagaGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCa                                                                                      >  1:800230/1‑91 (MQ=255)     agTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCAc                                                                                  >  2:408204/1‑91 (MQ=255)      gTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCAcc                                                                                 >  2:400393/1‑91 (MQ=255)        tCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCAt                                                                               >  2:842477/1‑91 (MQ=255)             gAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGc                                                                          <  1:104605/91‑1 (MQ=255)              aGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCa                                                                         <  1:108766/91‑1 (MQ=255)              aGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCa                                                                         >  1:735220/1‑91 (MQ=255)                   gcACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAAcc                                                                    <  1:408204/91‑1 (MQ=255)                   gcACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAAcc                                                                    <  1:688/91‑1 (MQ=255)                       gTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGAt                                                                <  1:594910/91‑1 (MQ=255)                       gTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGAt                                                                <  2:151963/91‑1 (MQ=255)                       gTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGAt                                                                <  1:190516/91‑1 (MQ=255)                              cGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTAc                                                         >  1:732479/1‑91 (MQ=255)                                tGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGa                                                       >  1:79062/1‑91 (MQ=255)                                 ggTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGAt                                                      <  1:400393/91‑1 (MQ=255)                                  gTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATc                                                     >  1:1099/1‑91 (MQ=255)                                  gTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATc                                                     >  2:735515/1‑91 (MQ=255)                                            aaaGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGt                                           >  2:193003/1‑91 (MQ=255)                                                            tttCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGtgat                           >  2:34985/1‑91 (MQ=255)                                                            tttCCGCGAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGtgat                           >  1:6306/1‑91 (MQ=255)                                                                 gcgAGATGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGt                      >  2:315699/1‑91 (MQ=255)                                                                   gagaTGTGGCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGa                    >  2:33321/1‑91 (MQ=255)                                                                          ggCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGa             <  2:1099/91‑1 (MQ=255)                                                                          ggCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGa             >  2:175624/1‑91 (MQ=255)                                                                          ggCGCAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGa             <  2:399245/91‑1 (MQ=255)                                                                             gcAAACTGGTAGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAg          >  2:669586/1‑91 (MQ=255)                                                                              cAAACTGGTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGc         <  2:49848/91‑1 (MQ=255)                                                                                    ggTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTAc   <  1:193003/91‑1 (MQ=255)                                                                                    ggTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTAc   >  2:839378/1‑91 (MQ=255)                                                                                    ggTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTAc   <  1:662262/91‑1 (MQ=255)                                                                                     gTCGGCATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACa  >  2:600157/1‑91 (MQ=255)                                                                                         |                                                                                       GAAGAGTTCGCCGAGCGCGCACGTAACTACGTGGTAAAACTGCAAAGCGGTGACGAATATTTCCGCGAGATGTGGCGCAAACTGGTCGACATCACCATGACGCAGAACCAGATCACCTACGATCGTCTCAACGTGACGCTGACCCGTGATGACGTGATGGGCGAAAGCCTCTACA  >  NZ_CP009273/1954928‑1955102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑