| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,000,880 | A→G | D415G (GAC→GGC) | amyA → | alpha‑amylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,000,880 | 0 | A | G | 100.0% | 82.4 / NA | 23 | D415G (GAC→GGC) | amyA | alpha‑amylase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/10); total (13/10) | |||||||||||
ATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGACCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGA > NZ_CP009273/2000795‑2000961 | atagataTGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATc < 1:775109/91‑1 (MQ=255)atagataTGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATc < 1:605500/91‑1 (MQ=255) tGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTg > 1:172608/1‑91 (MQ=255) tGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTg > 1:596272/1‑91 (MQ=255) cAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCAt < 1:39335/91‑1 (MQ=255) cAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCAt < 1:582149/91‑1 (MQ=255) gAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTa > 2:532955/1‑91 (MQ=255) gTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCAcc > 1:215502/1‑91 (MQ=255) ttCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACcgacga < 2:808981/91‑1 (MQ=255) gCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCAtt > 1:321646/1‑60 (MQ=255) gCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCAtt < 2:321646/60‑1 (MQ=255) tCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGc < 1:532955/91‑1 (MQ=255) tACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTg > 1:624665/1‑73 (MQ=255) tACAGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTg < 2:624665/73‑1 (MQ=255) aGACGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACgg > 1:521499/1‑91 (MQ=255) aCGTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACgggg > 1:838514/1‑91 (MQ=255) gTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCAcc < 2:291529/49‑1 (MQ=255) gTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCAcc > 1:291529/1‑49 (MQ=255) gTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGgat > 1:87155/1‑91 (MQ=255) gTTATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGgat > 2:423355/1‑91 (MQ=255) tATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCa > 1:316398/1‑75 (MQ=255) tATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCa < 2:316398/75‑1 (MQ=255) tATTTTTCGGCCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGgatga > 1:410620/1‑91 (MQ=255) | ATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTCGCCCGTCAGCGTTTCGCCCACGGTGTACAGACGTTATTTTTCGACCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCACCGACGAATTTCCCGGCTGCGTGGTGGTCATGTCGAACGGGGATGA > NZ_CP009273/2000795‑2000961 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |