Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,039,724	A→G	T436A (ACA→GCA)	yeeJ →	inverse autotransporter adhesin YeeJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,039,724	0	A	G	100.0%	117.8 / NA	32	T436A (ACA→GCA)	yeeJ	inverse autotransporter adhesin YeeJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/12);  total (20/12)

TTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGACAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAA  >  NZ_CP009273/2039638‑2039806                                                                                       |                                                                                   ttGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGa                                                                                >  1:586654/1‑91 (MQ=255)   gcaggcagCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTNNNAGAcc                                                                              <  2:477832/91‑1 (MQ=255)   gcaggcagCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGAcc                                                                              >  2:745384/1‑91 (MQ=255)      ggcagCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAg                                                                                 <  2:23378/85‑1 (MQ=255)      ggcagCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAg                                                                                 >  1:23378/1‑85 (MQ=255)          gCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGAc                                                                       >  1:725501/1‑91 (MQ=255)           ccGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACa                                                                      >  1:272767/1‑91 (MQ=255)               tATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGa                                                                  <  1:212866/91‑1 (MQ=255)               tATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGa                                                                  <  1:857847/91‑1 (MQ=255)                    tCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAg                                                                                 <  2:132541/71‑1 (MQ=255)                    tCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAg                                                                                 >  1:132541/1‑71 (MQ=255)                       ggtggATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGga                                                          >  2:695094/1‑91 (MQ=255)                       ggtggATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGga                                                          >  1:666240/1‑91 (MQ=255)                        gtggATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGgag                                                         >  1:232526/1‑91 (MQ=255)                            aTCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGt                                                     >  1:793446/1‑91 (MQ=255)                             tCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTg                                                    >  2:360308/1‑91 (MQ=255)                             tCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTg                                                    >  2:809973/1‑91 (MQ=255)                               gCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGaa                                                  >  2:500193/1‑91 (MQ=255)                               gCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGaa                                                  >  1:683520/1‑91 (MQ=255)                                 aacaacaaTATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAAt                                                >  1:97549/1‑91 (MQ=255)                                   caacaaTATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCa                                              <  2:38923/91‑1 (MQ=255)                                          atCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGttt                                       >  1:762741/1‑91 (MQ=255)                                             gTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTtcgt                                    >  2:156895/1‑91 (MQ=255)                                             gTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTtcgt                                    >  2:757146/1‑91 (MQ=255)                                               tCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGgag                                                         >  1:704139/1‑68 (MQ=255)                                               tCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGgag                                                         <  2:704139/68‑1 (MQ=255)                                                       atCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAAc                          <  1:727747/91‑1 (MQ=255)                                                       atCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAAc                          >  2:727747/1‑91 (MQ=255)                                                        tCGCAAAAAAGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAAcc                         >  1:737984/1‑91 (MQ=255)                                                                aaGACCTTGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGc                 <  2:47477/91‑1 (MQ=255)                                                                aaGAACTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGc                 <  1:210591/91‑1 (MQ=255)                                                                     cTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGa            <  2:259184/91‑1 (MQ=255)                                                                     cTGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGa            <  1:262842/91‑1 (MQ=255)                                                                      tGGTTCGCCTGACCCTGGCAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGaa           <  2:339762/91‑1 (MQ=255)                                                                               tgaccctggcAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATaa  <  2:302564/91‑1 (MQ=255)                                                                                       |                                                                                   TTGCAGGCAGCCGTTATGATCTGGTGGATCGCAACAACAATATCGTTCTGGAATATCGCAAAAAAGAACTGGTTCGCCTGACCCTGACAGACCCCGTGACAGGGAAGTCAGGAGAAGTGAAATCACTGGTTTCGTCGCTACAAACCAAATATGCCCTGAAAGGCTATAA  >  NZ_CP009273/2039638‑2039806

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑