Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,066,165	A→G	G382G (GGA→GGG)	agn43 →	autotransporter adhesin Ag43

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,066,165	0	A	G	100.0%	116.1 / NA	31	G382G (GGA→GGG)	agn43	autotransporter adhesin Ag43
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/14);  total (17/14)

CGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCA  >  NZ_CP009273/2066078‑2066251                                                                                         |                                                                                       cGGACACACAGCCACTAATGCCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAAt                                                                                     <  2:465829/91‑1 (MQ=255) cGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAAt                                                                                     <  1:321757/91‑1 (MQ=255) cGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAAt                                                                                     <  1:116653/91‑1 (MQ=255)    acacacAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGc                                                                                  <  2:11197/91‑1 (MQ=255)    acacacAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGc                                                                                  <  1:489467/91‑1 (MQ=255)    acacacAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGc                                                                                  <  1:626248/91‑1 (MQ=255)       cacaGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGgt                                                                               >  1:700104/1‑91 (MQ=255)        acaGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGgtg                                                                              >  2:830863/1‑91 (MQ=255)               cTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGGTGTActgct                                                                       >  2:11710/1‑91 (MQ=255)                 aaTACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGGTGTACTGCTgg                                                                     >  1:841731/1‑91 (MQ=255)                     cccGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGGTGTACTGCTGGCCGa                                                                 >  2:387812/1‑91 (MQ=255)                       cgcgTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCCATGGGGAATGGCGGTGTACTGCTGGCCGAt                                                                >  2:721411/1‑91 (MQ=255)                       cgcgTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGGTGTACTGCTGGCCGAtt                                                               >  1:484929/1‑91 (MQ=255)                        gcgTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGGTGTACTGCTGGCCGATTc                                                              >  2:291803/1‑91 (MQ=255)                        gcgTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGGTGTACTGCTGGCCGATTc                                                              >  1:542750/1‑91 (MQ=255)                         cgTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGGTGTACTGCTGGCCGATTcc                                                             >  1:260379/1‑91 (MQ=255)                         cgTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CC‑‑GGGGAATGGCGGTGTACTGCTGGCCGATTCCgg                                                           <  1:660774/91‑1 (MQ=255)                              atgatgGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGc                                                        <  1:33326/91‑1 (MQ=255)                                 atgGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGc                                                     >  2:508123/1‑91 (MQ=255)                                 atgGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGc                                                     >  2:727983/1‑91 (MQ=255)                                  tgGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTATCCCATGGGGAATGGCGGTGTACTGCTCGCCGATTCCGGTGCCGc                                                     <  1:721411/91‑1 (MQ=255)                                       gAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAg                                               >  1:670163/1‑91 (MQ=255)                                         aCGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTg                                             <  1:255158/91‑1 (MQ=255)                                                    ccGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGc                                  <  2:823400/91‑1 (MQ=255)                                                         aCGGTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACgg                             <  1:349213/91‑1 (MQ=255)                                                            gTGGCACCGCCACCACCGTAT‑CCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGaaa                          >  1:325664/1‑91 (MQ=255)                                                                   cGCCACCACCGTAT‑CCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTc                   <  2:521654/91‑1 (MQ=255)                                                                     ccaccaCCGTAT‑CCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAg                 <  2:260379/91‑1 (MQ=255)                                                                               aT‑CCATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAgga       >  1:76770/1‑90 (MQ=255)                                                                                   cATGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGt    >  2:687151/1‑91 (MQ=255)                                                                                     tGGGGAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCa  >  1:611669/1‑91 (MQ=255)                                                                                         |                                                                                       CGGACACACAGCCACTAATACCCGCGTGGATGATGGCGGAACGCTGGATGTCCGCAACGGTGGCACCGCCACCACCGTAT‑CCATGGGAAATGGCGGTGTACTGCTGGCCGATTCCGGTGCCGCTGTCAGTGGTACCCGGAGCGACGGAAAGGCATTCAGTATCGGAGGCGGTCA  >  NZ_CP009273/2066078‑2066251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑