Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,069,946	A→G	Y53C (TAT→TGT)	yeeS →	RadC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,069,946	0	A	G	100.0%	101.9 / NA	29	Y53C (TAT→TGT)	yeeS	RadC family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (14/15);  total (14/15)

GTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGA  >  NZ_CP009273/2069861‑2070032                                                                                      |                                                                                       gTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGa                                                                                   <  2:394012/91‑1 (MQ=255)  tGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGaa                                                                                  <  2:767735/91‑1 (MQ=255)             tCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATc                                                                       <  2:765620/91‑1 (MQ=255)              ccACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCa                                                                      >  2:830159/1‑91 (MQ=255)                aCCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGc                                                                    >  1:312261/1‑91 (MQ=255)                   cGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGa                                                                 >  2:183525/1‑91 (MQ=255)                   cGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTCGAGCGGGAAGAATTCCGGGTGCTGTGGCTGCACAAACAGAATCAGCTGa                                                                 >  2:179465/1‑91 (MQ=255)                      gcagcaCGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTg                                                              <  1:768534/91‑1 (MQ=255)                           aCGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGGGTCTGAACAACCAGAATCAGCTGATTGCgggg                                                         >  2:262541/1‑90 (MQ=37)                                        aTTCTGAACATGGCGGGACTGGAGCGGGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCa                                            >  2:754860/1‑91 (MQ=255)                                             gAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGc                                       >  2:410679/1‑91 (MQ=255)                                                cATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGATTCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCaat                                    <  1:802313/91‑3 (MQ=255)                                                 aTGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCa                                   <  1:122541/91‑1 (MQ=255)                                                 aTGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCa                                   <  2:312261/91‑1 (MQ=255)                                                  tGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCAt                                  >  1:358340/1‑91 (MQ=255)                                                  tGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCAt                                  >  2:141198/1‑91 (MQ=255)                                                  tGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCAt                                  >  2:588508/1‑91 (MQ=255)                                                  tGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCAt                                  >  2:485605/1‑91 (MQ=255)                                                   ggCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATc                                 >  1:6451/1‑91 (MQ=255)                                                             gAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACgg                       <  1:477702/91‑1 (MQ=255)                                                             gAGCGTGAAGAATTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACgg                       <  1:455798/91‑1 (MQ=255)                                                                     agaaTTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCAt               <  1:179465/91‑1 (MQ=255)                                                                     agaaTTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCAt               <  1:183525/91‑1 (MQ=255)                                                                     agaaTTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCAt               <  1:488823/91‑1 (MQ=255)                                                                        aTTCCGGGTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATccc            >  1:86110/1‑91 (MQ=255)                                                                             gggTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGa       <  1:739373/91‑1 (MQ=255)                                                                               gTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAAcc                                                   >  2:411931/1‑45 (MQ=255)                                                                               gTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAAcc                                                   <  1:411931/45‑1 (MQ=255)                                                                               gTGCTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAg     <  2:358340/91‑1 (MQ=255)                                                                                  cTGTGTCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGa  >  2:797593/1‑91 (MQ=255)                                                                                      |                                                                                       GTGGCCTTCACCTCCACCCGTGCAGCACGGGAATGGCTGATTCTGAACATGGCGGGACTGGAGCGTGAAGAATTCCGGGTGCTGTATCTGAACAACCAGAATCAGCTGATTGCCGGTGAAACCCTCTTCACCGGCACCATCAACCGCACGGAAGTCCATCCCCGGGAAGTGA  >  NZ_CP009273/2069861‑2070032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑