Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,136,211	A→G	D73D (GAT→GAC)	udk ←	uridine kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,136,211	0	A	G	100.0%	132.0 / NA	36	D73D (GAT→GAC)	udk	uridine kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/20);  total (16/20)

GCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTC  >  NZ_CP009273/2136126‑2136291                                                                                      |                                                                                 gcgTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCAt                                                                             <  2:390618/91‑1 (MQ=255)     aTGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATcgcg                                                                         >  2:540858/1‑91 (MQ=255)      tGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCgcgc                                                                        <  2:177052/91‑1 (MQ=255)       gTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCt                                                                       <  1:766862/91‑1 (MQ=255)           aaCATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGa                                                                   <  1:870996/91‑1 (MQ=255)            aCATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGAt                                                                  <  1:162613/91‑1 (MQ=255)            aCATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGAt                                                                  <  1:772735/91‑1 (MQ=255)            aCATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGAt                                                                  <  1:525632/91‑1 (MQ=255)            aCATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGAt                                                                  >  2:194819/1‑91 (MQ=255)             cATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATg                                                                 <  2:804021/91‑1 (MQ=255)                 gCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCg                                                             <  1:497804/91‑1 (MQ=255)                 gCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCg                                                             >  2:294487/1‑91 (MQ=255)                 gCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCg                                                             >  1:844353/1‑91 (MQ=255)                      aaaCCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGAt                                                                  >  1:683975/1‑81 (MQ=255)                      aaaCCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGAt                                                                  <  2:683975/81‑1 (MQ=255)                      aaaCCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGtt                                                        >  1:235929/1‑91 (MQ=255)                              aGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGAt                                                                  <  2:559156/73‑1 (MQ=255)                              aGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGAt                                                                  >  1:559156/1‑73 (MQ=255)                                         cGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTcc                                     >  1:474338/1‑91 (MQ=255)                                         cGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTcc                                     >  1:631260/1‑91 (MQ=255)                                            gCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATc                                  >  1:218821/1‑91 (MQ=255)                                                   ttCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGAt                           <  2:372522/91‑1 (MQ=255)                                                   ttCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGAt                           <  2:843629/91‑1 (MQ=255)                                                            tGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGAt                  >  1:509777/1‑91 (MQ=255)                                                            tGTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGAt                  >  1:256999/1‑91 (MQ=255)                                                             gTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATc                 <  2:379743/91‑1 (MQ=255)                                                             gTAAATGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATc                 <  1:348289/91‑1 (MQ=255)                                                                aaTGCTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCttt              <  2:154021/91‑1 (MQ=255)                                                                    cTCAAGCAGCAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTaa          >  1:331734/1‑91 (MQ=255)                                                                        agcagcAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGttcttc                                      <  2:386998/59‑1 (MQ=255)                                                                        agcagcAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGttcttc                                      >  1:386998/1‑59 (MQ=255)                                                                        agcagcAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGc      <  2:220027/91‑1 (MQ=255)                                                                        agcagcAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGc      <  2:558535/91‑1 (MQ=255)                                                                        agcagcAGACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGc      <  2:869788/91‑1 (MQ=255)                                                                            gcagACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTc  >  1:427076/1‑91 (MQ=255)                                                                            gcagACTGTGGTCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTc  >  1:356477/1‑91 (MQ=255)                                                                                      |                                                                                 GCGTATGTTCAACATAGCTGTAAACCGGCAGGTCAATTGCCGAGCCGCGTTTCAACGCTTGTAAATGCTCAAGCAGCAGACTGTGATCCATCGCGCTGGGATGGTCGTAGTTGGTCTTAACGCGTTCTTCCATCGACAGATGGCTTTGATCTTTGTAATAGCAGTC  >  NZ_CP009273/2136126‑2136291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑