Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	250,930	A→G	I425T (ATC→ACC)	pepD ←	cytosol nonspecific dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	250,930	0	A	G	100.0%	129.2 / NA	34	I425T (ATC→ACC)	pepD	cytosol nonspecific dipeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/13);  total (21/13)

GGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAG  >  NZ_CP009273/250842‑251014                                                                                         |                                                                                     ggTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTg                                                                                    >  1:689968/1‑91 (MQ=255)  gTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGgtgt                                                                                   >  1:520595/1‑91 (MQ=255)         ttGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGt                                                                            <  1:164077/91‑1 (MQ=255)           ggCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCt                                                                          >  2:79001/1‑91 (MQ=255)           ggCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCt                                                                          >  1:261208/1‑91 (MQ=255)           ggCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCt                                                                          <  1:79001/91‑1 (MQ=255)                   aGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAAc                                                                  <  2:696909/91‑1 (MQ=255)                     aaaCCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAg                                                                <  1:696302/91‑1 (MQ=255)                       aCCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGgc                                                              <  2:423342/91‑1 (MQ=255)                         cATGTGCATTTCCGGATACGGTGGTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGGGTTCGGCGTCTTGTTGAACAGgcgc                                                            <  2:132913/91‑1 (MQ=255)                         cATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGgcgc                                                            <  1:694700/91‑1 (MQ=255)                          aTGTACATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCt                                                           >  1:700873/1‑91 (MQ=255)                           tGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTg                                                          >  2:646931/1‑91 (MQ=255)                           tGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTg                                                          >  1:93790/1‑91 (MQ=255)                             tCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGAt                                                        <  2:392206/91‑1 (MQ=255)                                 tttCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGt                                                    <  1:490400/91‑1 (MQ=255)                                      ggATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCAc                                               >  2:749533/1‑91 (MQ=255)                                      ggATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCAc                                               <  2:706104/91‑1 (MQ=255)                                      ggATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCAc                                               >  2:643296/1‑91 (MQ=255)                                           cGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTAcc                                          >  2:849154/1‑91 (MQ=255)                                               tttttGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGAt                                      <  2:562401/91‑1 (MQ=255)                                                         gACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCgg                            >  1:139065/1‑91 (MQ=255)                                                         gACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCgg                            >  2:479659/1‑91 (MQ=255)                                                         gACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCgg                            >  1:184375/1‑91 (MQ=255)                                                         gACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCgg                            >  1:176395/1‑91 (MQ=255)                                                         gACCACATTCCAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCgg                            >  1:618911/1‑91 (MQ=255)                                                                   cAGGCCCGCGTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCg                  >  2:487436/1‑91 (MQ=255)                                                                          gcgTGGATACTCTGGGAGTTGGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCt           >  2:398358/1‑91 (MQ=255)                                                                          gcgTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCt           >  2:371377/1‑91 (MQ=255)                                                                          gcgTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCt           >  2:554925/1‑91 (MQ=255)                                                                          gcgTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCt           >  1:607100/1‑91 (MQ=255)                                                                          gcgTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGACGCTGATAGGTTTCACGTACAAGATGCATCACCGGAGAATTAGCGTCCGGCt           >  2:273952/1‑91 (MQ=255)                                                                            gTGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgc         <  1:769772/91‑1 (MQ=255)                                                                             tGGATAATCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTgcc        <  1:502902/91‑1 (MQ=255)                                                                                   aTCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGccagccag  >  2:679719/1‑91 (MQ=255)                                                                                   aTCTGGGTGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGccagccag  >  1:617201/1‑91 (MQ=255)                                                                                         |                                                                                     GGTGATAGTTGGCCCGATAGAAACCATGTCCATTTCCGGATACGGTTTTTTGAACAGACCACATTCCAGGCCCGCGTGGATAATCTGGATGTTCGGCGTCTTGTTGAACAGGCGCTGATAGGTTTCACGTACCAGATGCATCACCGGAGAATTAGCGTCCGGCTGCCAGCCAG  >  NZ_CP009273/250842‑251014

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑