Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,258,402 T→A I354N (ATC→AAC)  setB → sugar efflux transporter SetB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,258,4020TA100.0% 157.3 / NA 41I354N (ATC→AAC) setBsugar efflux transporter SetB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (23/18);  total (23/18)

TGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATGGTGATGATT  >  NZ_CP009273/2258321‑2258481
                                                                                 |                                                                               
tGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCCACACTTCGCGCGTGGGCTGGAACATCGCAgg                                                                        <  1:803143/91‑1 (MQ=255)
 gCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGa                                                                       >  1:57671/1‑91 (MQ=255)
   tctATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATc                                                                     >  2:557959/1‑91 (MQ=255)
                tCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCAtc                                                        <  2:842622/91‑1 (MQ=255)
                          ggTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCgaga                                              >  1:288380/1‑91 (MQ=255)
                             cAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCt                                           <  2:779122/91‑1 (MQ=255)
                             cAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCt                                           <  1:479903/91‑1 (MQ=255)
                              aGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTg                                          >  1:554930/1‑91 (MQ=255)
                                  gggTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAAt                                      <  1:556658/91‑1 (MQ=255)
                                     ttCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATc                                                                     <  2:824188/57‑1 (MQ=255)
                                     ttCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATc                                                                     >  1:824188/1‑57 (MQ=255)
                                       cAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCa                                 <  1:146934/91‑1 (MQ=255)
                                       cAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCa                                 <  2:664855/91‑1 (MQ=255)
                                         gCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCAACAGCGCCCAGATCTGGAGTTCTCAcc                               >  2:33449/1‑90 (MQ=255)
                                          ccaccaCGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGc                              <  1:557959/91‑1 (MQ=255)
                                          ccaccaCGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGc                              <  2:437818/91‑1 (MQ=255)
                                          ccaccaCGCTCTATACCAACACTGCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGc                              <  1:754564/91‑1 (MQ=255)
                                            accacGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCtg                            <  1:740543/91‑1 (MQ=255)
                                             ccacGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCtgt                           >  1:42155/1‑91 (MQ=255)
                                             ccacGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCtgt                           >  1:101791/1‑91 (MQ=255)
                                              cacGCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCtgtg                          >  1:536166/1‑91 (MQ=255)
                                                 gCTCTATACCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTc                       <  2:479587/91‑1 (MQ=255)
                                                      ataCCAACACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGtt                  <  1:465885/91‑1 (MQ=255)
                                                           aaCACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGc                              >  1:337917/1‑74 (MQ=255)
                                                           aaCACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGc                              <  2:337917/74‑1 (MQ=255)
                                                           aaCACTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGa             <  1:253321/91‑1 (MQ=255)
                                                            acacTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGCGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGAt            >  1:726070/1‑91 (MQ=255)
                                                            acacTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGAt            >  1:579803/1‑91 (MQ=255)
                                                            acacTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGAt            >  2:351575/1‑91 (MQ=255)
                                                              acTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTg                                                                 >  2:486804/1‑36 (MQ=38)
                                                              acTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTg                                                                 <  1:486804/36‑1 (MQ=38)
                                                              acTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGGATCGTCGCCGAGATCTGGAATTATCCCGCTGGTTTCTGGTTTTCGATgg          >  2:784961/1‑91 (MQ=255)
                                                              acTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGGATTATCACGCTGTGTTCTGGTTTGCGATgg          >  1:567622/1‑91 (MQ=255)
                                                              acTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATgg          >  1:761282/1‑91 (MQ=255)
                                                              acTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATgg          >  2:513007/1‑91 (MQ=255)
                                                              acTTCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATgg          >  1:398884/1‑91 (MQ=255)
                                                                ttCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGGATTATCACGCTGTGTTCTGGTTTGCGGTgggg        >  2:98639/1‑89 (MQ=255)
                                                                ttCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTTGTTTGCGATgggg        >  1:27072/1‑89 (MQ=255)
                                                                ttCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATGGtg        >  1:267702/1‑91 (MQ=255)
                                                                 tCGCGCGTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATGGtga       >  1:474530/1‑91 (MQ=255)
                                                                      cgTGGGCTGGAACATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATGGTGATGAtt  <  1:513007/91‑1 (MQ=255)
                                                                                 |                                                                               
TGCTCTATTTTCAGGATCTGATGCCCGGTCAGGCGGGTTCAGCCACCACGCTCTATACCAACACTTCGCGCGTGGGCTGGATCATCGCAGGATCAGTGGCGGGCATCGTCGCCGAGATCTGGAATTATCACGCTGTGTTCTGGTTTGCGATGGTGATGATT  >  NZ_CP009273/2258321‑2258481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑