Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,268,854	A→G	S35G (AGT→GGT)	yejE →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,268,854	0	A	G	100.0%	83.5 / NA	23	S35G (AGT→GGT)	yejE	ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/12);  total (11/12)

CCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGAGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAA  >  NZ_CP009273/2268767‑2268935                                                                                        |                                                                                  cccGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGtt                                                                                <  1:738064/91‑1 (MQ=255)   cGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTggtttgggtttg                                                                              <  1:634797/91‑1 (MQ=255)   cGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTggtttgggtttg                                                                              <  2:166136/91‑1 (MQ=255)    gTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTtgt                                                                             >  2:29786/1‑91 (MQ=255)                 gTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTAt                                                                >  2:636445/1‑91 (MQ=255)                     ggCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGcc                                                            <  2:665461/91‑1 (MQ=255)                      gcgcGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCa                                                           >  2:626872/1‑91 (MQ=255)                          gTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGa                                                       >  2:264565/1‑91 (MQ=255)                                   aTAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGtt                                              <  2:535971/91‑1 (MQ=255)                                         gTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGt                                        >  2:767181/1‑91 (MQ=255)                                          tCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTg                                       >  1:31644/1‑91 (MQ=255)                                              ggCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGtt                                   <  1:336782/91‑1 (MQ=255)                                                    tGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACg                             <  1:780360/91‑1 (MQ=255)                                                     ggTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCg                                     >  2:320081/1‑82 (MQ=255)                                                     ggTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCg                                     <  1:320081/82‑1 (MQ=255)                                                         gTTATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGt                        >  1:64815/1‑91 (MQ=255)                                                          ttATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGtt                       <  1:622322/91‑1 (MQ=255)                                                           tATGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTg                      <  1:866036/91‑1 (MQ=255)                                                            aTGGATTTTCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTgg                     <  1:626872/91‑1 (MQ=255)                                                                   ttCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTcc              >  2:555894/1‑91 (MQ=255)                                                                   ttCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTcc              >  1:208957/1‑91 (MQ=255)                                                                    tCCTCGTCTTGTTTGGTTTGGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTccc             <  1:264565/91‑1 (MQ=255)                                                                              gtttggtttgGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGaaa   <  2:84352/91‑1 (MQ=255)                                                                               tttggtttggGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGaaaa  <  2:31644/91‑1 (MQ=255)                                                                                   gtttgGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGt                        >  2:111973/1‑65 (MQ=255)                                                                                   gtttgGGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGt                        <  1:111973/65‑1 (MQ=255)                                                                                        |                                                                                  CCCGTCAATCAGGCCCGTTGGGCGCGTTTTCGTCATAACCGTCGCGGCTACTGGTCGTTATGGATTTTCCTCGTCTTGTTTGGTTTGAGTTTGTGTTCTGAACTTATCGCCAACGATAAACCGTTGCTGGTGCGTTATGACGGCAGTTGGTATTTCCCGTTATTGAAAA  >  NZ_CP009273/2268767‑2268935

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑