Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,357,677	A→G	G26G (GGT→GGC)	yfaZ ←	YfaZ family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,357,677	0	A	G	100.0%	123.4 / NA	33	G26G (GGT→GGC)	yfaZ	YfaZ family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/13);  total (20/13)

CGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCT  >  NZ_CP009273/2357589‑2357762                                                                                         |                                                                                      cgtTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGcc                                                                                     <  1:591043/91‑1 (MQ=255)       gtgtCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGAt                                                                               >  1:329948/1‑91 (MQ=255)        tgtCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATg                                                                              <  1:339332/91‑1 (MQ=255)         gtCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGc                                                                             >  2:246015/1‑91 (MQ=255)            cAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTca                                                                          <  1:246015/91‑1 (MQ=255)            cAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTca                                                                          <  1:523121/91‑1 (MQ=255)             aGTTACCGCTTCACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTcat                                                                         >  2:869756/1‑91 (MQ=255)             aGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTcat                                                                         <  1:710290/91‑1 (MQ=255)              gTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTcatt                                                                        >  2:579916/1‑91 (MQ=255)                   cGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTcattgcatt                                                                   >  1:807530/1‑91 (MQ=255)                    gCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTcattgcattg                                                                  >  2:295249/1‑91 (MQ=255)                        aaCGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGAcc                                                               >  2:327253/1‑90 (MQ=255)                        aaCGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGAcc                                                               <  1:327253/90‑1 (MQ=255)                        aaCGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCg                                                              >  1:835306/1‑91 (MQ=255)                              aGGCCCGTCGTTTCAGTTCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAg                                                        >  2:803166/1‑91 (MQ=255)                              aGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAg                                                        >  1:654569/1‑91 (MQ=255)                              aGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAg                                                        <  2:316981/91‑1 (MQ=255)                              aGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAg                                                        >  1:818632/1‑91 (MQ=255)                                   cgtcgtTTCAGTACCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAAcc                                                   >  2:740544/1‑91 (MQ=255)                                         ttCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGc                                             >  2:8080/1‑91 (MQ=255)                                             gTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTc                                         <  2:327067/91‑1 (MQ=255)                                             gTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTc                                         <  2:518507/91‑1 (MQ=255)                                                       cGACACCAATATTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGAcc                               >  1:729089/1‑91 (MQ=255)                                                                tatTGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAgcgc                      <  1:785231/91‑1 (MQ=255)                                                                  ttGGTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCaa                    <  1:864585/91‑1 (MQ=255)                                                                    ggTGTATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAAtt                  >  2:685152/1‑91 (MQ=255)                                                                      tgtATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAAtttt                >  2:261253/1‑91 (MQ=255)                                                                      tgtATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAAtttt                >  1:359371/1‑91 (MQ=255)                                                                      tgtATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAAtttt                >  1:145723/1‑91 (MQ=255)                                                                        tATTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAAtttttt              <  2:346454/91‑1 (MQ=255)                                                                         aTTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAAttttttt             <  1:8080/91‑1 (MQ=255)                                                                         aTTCTTTACCCGCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAAttttttt             >  2:84197/1‑91 (MQ=255)                                                                                    gCCTGGCCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCt  >  2:62283/1‑91 (MQ=255)                                                                                         |                                                                                      CGTTATGTGTCCAGTTACCGCTTAACGCCAGGCCCGTCGTTTCAGTCCCAAAACCGACACCAATATTGGTGTATTCTTTACCCGCCTGACCGCTGATGCTCATTGCATTGACCGATGCAGAAACCAACAGCATTCCGGCCAGACCTGCTAGCGCAATTTTTTTCATTATCCCCT  >  NZ_CP009273/2357589‑2357762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑