| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,370,881 | A→G | V64A (GTC→GCC) | menH ← | 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,370,881 | 0 | A | G | 100.0% | 52.4 / NA | 15 | V64A (GTC→GCC) | menH | 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (11/4); total (11/4) | |||||||||||
AAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCT > NZ_CP009273/2370811‑2370968 | aaGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACg > 1:509154/1‑91 (MQ=255)aaGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACg > 2:412093/1‑91 (MQ=255) gTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATc > 2:354862/1‑91 (MQ=255) agccagAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAac > 2:513011/1‑91 (MQ=255) gccagAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAacc > 1:157000/1‑91 (MQ=255) cAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCg < 1:329438/86‑1 (MQ=255) cAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCg > 2:329438/1‑86 (MQ=255) aTGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGga < 2:509154/91‑1 (MQ=255) tgtAACTAACCAAGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATc < 1:213088/91‑1 (MQ=255) aaGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAAcc > 2:109191/1‑91 (MQ=255) aaGGTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAAcc > 2:259690/1‑91 (MQ=255) gTTTTACGCAGTAAGTCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTg < 1:412093/91‑1 (MQ=255) tCGGTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGCGATCAACATACAACCGTGAGCAGTCCGCAAACg > 1:61777/1‑91 (MQ=255) gTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCt > 1:427148/1‑91 (MQ=255) gTGGCATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGAAAACGCCt > 2:142204/1‑91 (MQ=255) | AAGCGAGTACCCCACCAGCCAGAAGTCAAGGATGTTGTAACTAACCAAGGTTTTACGCAGTAAGTCGGTGACATCATCAAATCCATCGACGCTAATCGCCGCCGAACCACCGTGACCTGGGAGATCAACATACAACCGTGAGTAGTCGGCAAACGCCT > NZ_CP009273/2370811‑2370968 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |