Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,432,635	G→T	A322E (GCG→GAG)	yfcJ ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,432,635	0	G	T	100.0%	90.3 / NA	25	A322E (GCG→GAG)	yfcJ	MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/12);  total (13/12)

AAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAAC  >  NZ_CP009273/2432562‑2432721                                                                          |                                                                                       aaaCGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGaa                                                                       <  2:227972/91‑1 (MQ=255)     gcggcgTAACCGCCCAGTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATc                                                                   >  2:642220/1‑91 (MQ=255)     gcggcgTAACCGCCCAGTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATc                                                                   >  2:356039/1‑91 (MQ=255)       ggcgTAACCGCCCAGTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCg                                                                 >  1:451029/1‑91 (MQ=255)                     gTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAAcgc                                                   >  1:643218/1‑91 (MQ=255)                        cGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAAcgccgc                                                <  2:48703/91‑1 (MQ=255)                          gTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAAcgccgctc                                              >  2:780198/1‑89 (MQ=255)                              cgcgAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGc                                          <  2:752791/91‑1 (MQ=255)                               gcgAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCt                                         >  1:767492/1‑91 (MQ=255)                               gcgAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCt                                         >  2:485511/1‑91 (MQ=255)                               gcgAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCt                                         >  1:843196/1‑91 (MQ=255)                                   aCTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATg                                     <  1:14998/91‑1 (MQ=255)                                   aCTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATg                                     <  1:198218/91‑1 (MQ=255)                                    cTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGc                                    >  1:667064/1‑91 (MQ=255)                                           ggggACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCAt                             <  2:176541/91‑1 (MQ=255)                                           ggggACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCAt                             <  2:401344/91‑1 (MQ=255)                                           ggggACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCAt                             <  2:635354/91‑1 (MQ=255)                                            gggACGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATg                            <  2:339385/91‑1 (MQ=255)                                               aCGCGTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCAc                         >  1:83330/1‑91 (MQ=255)                                                 gcgTTTAACCACCTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCt                       >  2:434815/1‑91 (MQ=255)                                                           accTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGcc             <  1:86020/91‑1 (MQ=255)                                                            ccTCCACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCa            >  1:138539/1‑91 (MQ=255)                                                               ccACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCagag         <  1:843368/91‑1 (MQ=255)                                                                cACGCCCAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGagc        >  1:821576/1‑91 (MQ=255)                                                                      cAGCTCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGcaac  <  2:170552/91‑1 (MQ=255)                                                                          |                                                                                       AAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCGAACATCCGGCTCCGGTTAACGCCGCGCCCGCTAATGCGACCCATGCACCTGGGGCTTGCCAGAGCAGCAAC  >  NZ_CP009273/2432562‑2432721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑