Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	251,113	A→G	I364T (ATC→ACC)	pepD ←	cytosol nonspecific dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	251,113	0	A	G	100.0%	75.3 / NA	21	I364T (ATC→ACC)	pepD	cytosol nonspecific dipeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/12);  total (9/12)

TTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGATCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACAT  >  NZ_CP009273/251026‑251197                                                                                        |                                                                                     ttCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCa                                                                                   <  1:850286/91‑1 (MQ=255) ttCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCGGTCGATCAGTGAACGGGTCa                                                                                   >  1:846154/1‑91 (MQ=255)      ttCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAg                                                                              <  2:617201/91‑1 (MQ=255)         gtttttCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGa                                                                           <  2:255120/89‑1 (MQ=255)              tCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTc                                                                      <  2:3336/91‑1 (MQ=255)                                             cATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCg                                       <  1:371377/91‑1 (MQ=255)                                              aTGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGa                                      <  1:849154/91‑1 (MQ=255)                                                       acGTAGTCTTTACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAggg                             <  1:699286/91‑1 (MQ=255)                                                               tttACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAGTTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCa                     >  2:789281/1‑91 (MQ=255)                                                                 tACCGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAAc                   <  1:554925/91‑1 (MQ=255)                                                                   ccGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACca                 <  2:139065/91‑1 (MQ=255)                                                                    cGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACcac                <  1:67214/91‑1 (MQ=255)                                                                    cGCTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACcac                <  2:618911/91‑1 (MQ=255)                                                                      cTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCacac              >  1:458664/1‑91 (MQ=255)                                                                      cTGTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCacac              >  1:247142/1‑91 (MQ=255)                                                                        gTCGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCt            >  1:509954/1‑91 (MQ=255)                                                                          cGATCAGTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCttt          >  1:112216/1‑91 (MQ=255)                                                                                gTGAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTAc    <  2:458664/91‑1 (MQ=255)                                                                                  gAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACAt  >  2:101650/1‑91 (MQ=255)                                                                                  gAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACAt  >  2:572539/1‑91 (MQ=255)                                                                                  gAACGGGTCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACAt  >  2:572540/1‑91 (MQ=255)                                                                                        |                                                                                     TTCGCTTCGGTTTTCGCGCCAGCCAGTTTACCCAGCGAATCCAGCATGCTCACCACGTAGTCTTTACCGCTGTCGATCAGTGAACGGATCAGGCAGTGAATTTCTACGTTATTGTCAGTCATGGTCACCACACCGACGTTCAGGGAGGTTTCAACCACACCTTTGGCTACAT  >  NZ_CP009273/251026‑251197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑