Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,521,647	C→T	G630D (GGC→GAC)	ligA ←	NAD‑dependent DNA ligase LigA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,521,647	0	C	T	100.0%	82.1 / NA	23	G630D (GGC→GAC)	ligA	NAD‑dependent DNA ligase LigA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/7);  total (16/7)

CAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGCCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGT  >  NZ_CP009273/2521564‑2521732                                                                                    |                                                                                      cAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGAc                                                                                <  1:207129/91‑1 (MQ=255) cAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGAc                                                                                <  1:726811/91‑1 (MQ=255)        cAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTcgcg                                                                         >  1:591707/1‑91 (MQ=255)        cAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTCTCCGCGACTTTcgcg                                                                         >  1:869551/1‑91 (MQ=255)         aGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCgcgc                                                                        <  1:345510/91‑1 (MQ=255)               tGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGtt                                                                  >  2:221140/1‑91 (MQ=255)                   ccTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGAc                                                              <  2:632793/91‑1 (MQ=255)                    cTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTc                                                                            >  2:538757/1‑76 (MQ=255)                    cTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTc                                                                            <  1:538757/76‑1 (MQ=255)                    cTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGAcc                                                             >  1:479983/1‑91 (MQ=255)                    cTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGAcc                                                             >  2:345055/1‑91 (MQ=255)                            gTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGc                                                     >  1:1074/1‑91 (MQ=255)                                      tGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGtca                                           >  2:817498/1‑91 (MQ=255)                                      tGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGtca                                           >  2:342969/1‑91 (MQ=255)                                                  cGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATc                               <  2:479983/91‑1 (MQ=255)                                                  cGCTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATc                               <  2:495857/91‑1 (MQ=255)                                                    cTATCACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTg                             >  1:217015/1‑91 (MQ=255)                                                        cACCAGATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCtt                         >  1:1076/1‑91 (MQ=255)                                                             gATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCt                    >  2:833968/1‑91 (MQ=255)                                                             gATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCt                    >  2:833969/1‑91 (MQ=255)                                                             gATCGGTTTTCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTCCGACCAGTCGAGCTTTAGCGCCCTCACGCAACATCTGGCTTCAGCt                    >  2:665721/1‑91 (MQ=255)                                                                    tttCTTCGACACGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTa             >  1:371262/1‑91 (MQ=255)                                                                               cGCTGTCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGt  >  1:506176/1‑91 (MQ=255)                                                                                    |                                                                                      CAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGCCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGTCATCACGCGACATCTGGCTTAAGCTGCCCGTAAGCACCACGGT  >  NZ_CP009273/2521564‑2521732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑