Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,550,584	A→G	S25P (TCA→CCA)	eutC ←	ethanolamine ammonia‑lyase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,550,584	0	A	G	100.0%	122.7 / NA	33	S25P (TCA→CCA)	eutC	ethanolamine ammonia‑lyase subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/17);  total (16/17)

CTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATA  >  NZ_CP009273/2550497‑2550660                                                                                        |                                                                             cTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCgg                                                                           >  1:739434/1‑91 (MQ=255)              ggAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCt                                                              <  1:535183/91‑1 (MQ=255)                aaCCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTg                                                            >  2:227666/1‑91 (MQ=255)                 aCCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGt                                                           >  1:624858/1‑91 (MQ=255)                        tCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTg                                                    <  1:144407/91‑1 (MQ=255)                           aGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACg                                                 >  2:350844/1‑91 (MQ=255)                           aGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACg                                                 >  1:98579/1‑91 (MQ=255)                           aGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTCGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACg                                                 >  2:591809/1‑91 (MQ=255)                            gcgcACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGc                                                <  2:256236/91‑1 (MQ=255)                            gcgcACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGc                                                >  1:66381/1‑91 (MQ=255)                                    cTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGc                                        <  2:98579/91‑1 (MQ=255)                                    cTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGc                                        <  1:264254/91‑1 (MQ=255)                                    cTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGc                                        <  1:534978/91‑1 (MQ=255)                                      ttCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTg                                      <  2:436638/91‑1 (MQ=255)                                              tCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCCCGCTGCGTACAAt                              <  2:111620/91‑1 (MQ=255)                                                              gTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGtttt              >  1:392490/1‑91 (MQ=255)                                                              gTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGtttt              >  2:633690/1‑91 (MQ=255)                                                              gTTGGTGGTGGCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGtttt              >  2:500019/1‑91 (MQ=255)                                                                  gtggtggCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGAt          <  2:739434/91‑1 (MQ=255)                                                                   tggtggCGCACTTTGCTTCTGGCGACGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATc         <  2:705728/91‑1 (MQ=255)                                                                      tggCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCAt      >  1:188634/1‑91 (MQ=255)                                                                      tggCGCACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCAt      >  2:527527/1‑91 (MQ=255)                                                                        gcgcACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGa    >  2:583/1‑91 (MQ=255)                                                                        gcgcACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGa    >  1:211877/1‑91 (MQ=255)                                                                        gcgcACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGa    >  1:241500/1‑91 (MQ=255)                                                                         cgcACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGat   <  1:848595/91‑1 (MQ=255)                                                                         cgcACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGat   <  1:227666/91‑1 (MQ=255)                                                                         cgcACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGat   <  2:66381/91‑1 (MQ=255)                                                                         cgcACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGat   <  1:350844/91‑1 (MQ=255)                                                                         cgcACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGat   <  2:847364/91‑1 (MQ=255)                                                                          gcACTTTGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGata  <  2:91169/91‑1 (MQ=255)                                                                               ttGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTg                                                    <  2:411420/36‑1 (MQ=255)                                                                               ttGCTTCTGGCGGCGCGGGGGCCGCTTGTCCCATTg                                                    >  1:411420/1‑36 (MQ=38)                                                                                        |                                                                             CTTTTGCTTCAGCGGAACCTAAATCCAGCGCACAGCTTTCCGAGGTCACCGGTGCCGCACAGTTGGTGGTGGCGCACTTTGCTTCTGACGGCGCGGGGGCCGCTTGTCCCATTGACGCCATCACGCTGCGTACAATTTCTTCAATCTGTTTTTGATCCATGATA  >  NZ_CP009273/2550497‑2550660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑