Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,637,057	G→A	L196F (CTT→TTT)	rlmN ←	bifunctional tRNA (adenosine(37)‑C2)‑methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,637,057	0	G	A	100.0%	156.1 / NA	41	L196F (CTT→TTT)	rlmN	bifunctional tRNA (adenosine(37)‑C2)‑methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/24);  total (17/24)

TCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATC  >  NZ_CP009273/2636972‑2637136                                                                                      |                                                                                tCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAACGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGa                                                                            >  2:345199/1‑91 (MQ=255) tCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGa                                                                            >  1:166522/1‑91 (MQ=255)     ccAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTc                                                                        >  2:319256/1‑91 (MQ=255)           tATCCAGCGACGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGc                                                                  <  2:595947/91‑1 (MQ=255)            aTCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGcc                                                                 <  2:436708/91‑1 (MQ=255)            aTCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGcc                                                                 <  1:778585/91‑1 (MQ=255)                 gcgcCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAac                                                            <  2:499227/91‑1 (MQ=255)                 gcgcCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAac                                                            >  2:269030/1‑91 (MQ=255)                 gcgcCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAac                                                            >  2:185189/1‑91 (MQ=255)                  cgcCGGAACTACACCGGAAGTGGACCGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAacg                                                           >  2:124842/1‑91 (MQ=255)                  cgcCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAacg                                                           >  1:805251/1‑91 (MQ=255)                                 ggAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTg                                            >  2:215755/1‑91 (MQ=255)                                 ggAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTg                                            >  1:472154/1‑91 (MQ=255)                                     gTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGagca                                        <  1:369473/91‑1 (MQ=255)                                       ggAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGagcagc                                      <  2:774334/91‑1 (MQ=255)                                       ggAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGagcagc                                      >  1:300169/1‑91 (MQ=255)                                          aaGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGc                                   <  1:668954/91‑1 (MQ=255)                                           aGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCt                                  <  2:758964/91‑1 (MQ=255)                                           aGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCt                                  <  2:209859/91‑1 (MQ=255)                                            gCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTc                                 <  1:269030/91‑1 (MQ=255)                                            gCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTc                                 <  1:470165/91‑1 (MQ=255)                                                         tttAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCcatc                    <  2:37212/91‑1 (MQ=255)                                                          ttAGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCcatca                   <  1:480547/91‑1 (MQ=255)                                                            aGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCatcatc                 <  1:31310/91‑1 (MQ=255)                                                            aGACAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCatcatc                 <  2:144194/91‑1 (MQ=255)                                                               cAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACt              <  1:268050/91‑1 (MQ=255)                                                               cAGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACt              <  2:727560/91‑1 (MQ=255)                                                                aGGCCAAAACCGGAATCATCAAACATGATTTCCATCGCCGGAACGACGGTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTa             >  1:813480/1‑91 (MQ=255)                                                                aGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCatcatc                 <  1:651618/87‑1 (MQ=255)                                                                aGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCatcatc                 >  2:651618/1‑87 (MQ=255)                                                                aGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTa             >  2:763336/1‑91 (MQ=255)                                                                aGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTa             >  2:844363/1‑91 (MQ=255)                                                                aGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTa             <  1:844363/91‑1 (MQ=255)                                                                aGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTa             <  1:763430/91‑1 (MQ=255)                                                                aGGCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTa             <  1:185189/91‑1 (MQ=255)                                                                 ggCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCTATGCCCATCATCACTAc            >  2:90684/1‑91 (MQ=255)                                                                  gCCAAAACCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACg           >  1:277451/1‑91 (MQ=255)                                                                      aaaCCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCAc               <  1:318611/83‑1 (MQ=255)                                                                      aaaCCGAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCCCCCATGCCCATCATCAc               >  2:318611/1‑83 (MQ=255)                                                                           gAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATc  <  1:12439/91‑1 (MQ=255)                                                                           gAAATCATCAAACATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATc  <  1:340300/91‑1 (MQ=255)                                                                                      |                                                                                TCGCCCAGTTTATCCAGCGCCGGAACTACACCGGAAGTGGAAAGCGTGACGCGACGTTTAGACAGGCCAAAACCGAAATCATCAAGCATGATTTCCATCGCCGGAACGACGTTGTTCAGGTTGAGCAGCGGCTCGCCCATGCCCATCATCACTACGTTAGTGATC  >  NZ_CP009273/2636972‑2637136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑