Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,649,663	C→T	G19R (GGA→AGA)	pepB ←	aminopeptidase PepB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,649,663	0	C	T	100.0%	93.5 / NA	24	G19R (GGA→AGA)	pepB	aminopeptidase PepB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/6);  total (18/6)

CAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGG  >  NZ_CP009273/2649589‑2649743                                                                           |                                                                                 cAGCCCCAGATCGGCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAAGGCTGTAAGTTTCTTTTTCTCTCCAGCGCGCGTCGGCa                                                                  <  2:415320/91‑1 (MQ=255)   gCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAgg                                                                >  1:274202/1‑91 (MQ=255)             gTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTagag                                                      >  2:781468/1‑91 (MQ=255)             gTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTagag                                                      <  2:654842/91‑1 (MQ=255)             gTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTagag                                                      >  1:685366/1‑91 (MQ=255)              tCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTagaga                                                     <  1:171155/91‑1 (MQ=255)                  ccccGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGt                                                 <  2:7150/91‑1 (MQ=255)                       ttCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCt                                            <  2:324526/91‑1 (MQ=255)                                   gTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTg                                >  1:470125/1‑91 (MQ=255)                                    tAATGCCGTCATTAATAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGt                               <  2:353073/91‑1 (MQ=255)                                         ccGTCATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGT‑‑AGAGGGTAATCTTCATCGCTTCTGTCATTTTa                        >  1:92865/1‑91 (MQ=255)                                           gTCATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCAtt                           <  2:318225/88‑1 (MQ=255)                                           gTCATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCAtt                           >  1:318225/1‑88 (MQ=255)                                           gTCATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTa                        >  1:832320/1‑91 (MQ=255)                                             cATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGt                      >  2:271000/1‑91 (MQ=255)                                             cATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGt                      >  2:474993/1‑91 (MQ=255)                                             cATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGt                      >  2:556191/1‑91 (MQ=255)                                             cATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGt                      >  1:283209/1‑91 (MQ=255)                                             cATTATTAATGCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGt                      >  1:683170/1‑91 (MQ=255)                                                       gCTGTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCcttctt            >  1:67406/1‑91 (MQ=255)                                                          gTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGt         >  1:737889/1‑91 (MQ=255)                                                          gTAAGTTGCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGt         >  1:729720/1‑91 (MQ=255)                                                                 gCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAggg  >  1:657941/1‑91 (MQ=255)                                                                 gCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAggg  >  1:747971/1‑91 (MQ=255)                                                                 gCTTTTTCTCTCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAggg  >  2:498122/1‑91 (MQ=255)                                                                           |                                                                                 CAGCCCCAGATCGTCTGCCCCGTTCAAATGCAGGGTAATGCCGTCATTATTAATGCTGTAAGTTGCTTTTTCTCCCCAGCGCGCGTCGGCAGGTTGGGTAGAGAGGGTAATCTTCATCGCTTCTGTCATTTTAGTTATCCTTCTTTGTAAAAGGG  >  NZ_CP009273/2649589‑2649743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑